A MATING TYPE-LINKED MUTATION THAT DISRUPTS THE UNIPARENTAL INHERITANCE OF CHLOROPLAST DNA ALSO DISRUPTS CELL-SIZE CONTROL IN CHLAMYDOMONAS
A MATING TYPE-LINKED MUTATION THAT DISRUPTS THE UNIPARENTAL INHERITANCE OF CHLOROPLAST DNA ALSO DISRUPTS CELL-SIZE CONTROL IN CHLAMYDOMONAS
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DOI：
10.1091/mbc.6.12.1807
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发表时间：
1995-12-01
影响因子：
3.3
通讯作者：
GOODENOUGH, UW
中科院分区：
文献类型：
--
作者：
ARMBRUST, EV;IBRAHIM, A;GOODENOUGH, UW
莱茵衣藻早期受精卵发育的一个耐人寻味的特征是，可能由于受精卵特有的核酸酶的作用，叶绿体DNA从交配型负亲本中主动消除。因此，减数分裂后代几乎完全从交配型+亲本那里遗传叶绿体DNA。正连锁核突变mat3防止这种选择性破坏负的叶绿体DNA，并产生表现出双亲遗传模式的后代。在这里，我们表明mat3突变创造了以前没有描述过的其他表型：细胞比野生型小得多，它们拥有大量的线粒体和叶绿体DNA。我们认为，mat3突变的主要缺陷是细胞大小控制的中断，而叶绿体基因组单亲传递的抑制是mat3亲本叶绿体DNA数量减少的次要后果。
An intriguing feature of early zygote development in Chlamydomonas reinhardtii is the active elimination of chloroplast DNA from the mating-type minus parent due presumably to the action of a zygote-specific nuclease. Meiotic progeny thus inherit chloroplast DNA almost exclusively from the mating-type plus parent. The plus-linked nuclear mutation mat3 prevents this selective destruction of minus chloroplast DNA and generates progeny that display a biparental inheritance pattern. Here we show that the mat3 mutation creates additional phenotypes not previously described: the cells are much smaller than wild type and they possess substantially reduced amounts of both mitochondrial and chloroplast DNA. We propose that the primary defect of the mat3 mutation is a disruption of cell-size control and that the inhibition of the uniparental transmission of chloroplast genomes is a secondary consequence of the reduced amount of chloroplast DNA in the mat3 parent.