Immunization of rats against Fasciola hepatica using crude antigens conjugated with Freund's adjuvant or oligodeoxynucleotides
Immunization of rats against Fasciola hepatica using crude antigens conjugated with Freund's adjuvant or oligodeoxynucleotides
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DOI：
10.1016/j.vetimm.2003.08.015
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发表时间：
2004-01-01
影响因子：
1.8
通讯作者：
Masih, D
中科院分区：
文献类型：
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作者：
Cervi, L;Borgonovo, J;Masih, D
慢性肝片吸虫感染与辅助性T细胞（Th 2）为主的免疫反应的发展相关。为了确定已知促进Th 1（T辅助细胞1）免疫应答的免疫刺激性含CpG的寡脱氧核苷酸（CpG-ODN）或弗氏完全佐剂（FCA）是否可以提供针对肝片吸虫感染的保护，将肝片吸虫的总匀浆（TH）与CpG-ODN或FCA混合皮下（s.c.）Wistar大鼠。CpG-ODN和FCA均可诱导小鼠淋巴结产生Th 1型免疫应答。与来自仅注射TH的大鼠的淋巴细胞相比，来自TH-CpG-ODN或TH-FCA免疫的大鼠的淋巴结细胞显示出增加的抗原特异性增殖和高水平的INF γ。与此相反，这两组免疫动物没有修改IL-4释放引流淋巴结细胞，当他们随后刺激TH在体外。然而，仅在用TH-FCA免疫的大鼠中观察到吸虫负担的显著降低（76.7%）。相反，用TH-CpG-ODN免疫大鼠并不能促进对寄生虫的保护。因此，尽管CpG-ODNs和FCA诱导了Th 1型应答，但只有FCA对感染F.肝。(C)2003 Elsevier B. V.保留所有权利。
Chronic Fasciola hepatica infection is correlated with the development of a T helper (Th2)-predominant immune response. To determine whether immunostimulatory CpG-containing oligodeoxynucleotides (CpG-ODN) or Freund's complete adjuvant (FCA), known to promote a Th1 (T helper 1) immune responses, could provide protection from E hepatica infection, total homogenate (TH) of F hepatica mixed with CpG-ODN or FCA were injected subcutaneously (s.c.) into Wistar rats. A E hepatica-specific Th1-predominant immune response was induced with CpG-ODN or FCA in lymph nodes of immunized animals. Lymph node cells from TH-CpG-ODN or TH-FCA immunized rats showed increased antigen-specific proliferation with high levels of INFgamma, compared to lymphocytes from rats injected with TH alone. In contrast, these two groups of immunized animals did not modify IL-4 release by draining lymph node cells, when they were subsequently stimulated with TH in vitro. However, a significant reduction in the burden of flukes (76.7%) was only observed in rats immunized with TH-FCA. Conversely, immunization of rats with TH-CpG-ODN did not promote protection against the parasite. Therefore, even though CpG-ODNs and FCA induced Th1 type responses, only FCA provided a significant protection to rats infected with F. hepatica. (C) 2003 Elsevier B.V. All rights reserved.