Low-volume exercise training attenuates oxidative stress and neutrophils activation in older adults
Low-volume exercise training attenuates oxidative stress and neutrophils activation in older adults
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小容量运动训练可减轻老年人的氧化应激和中性粒细胞活化
DOI：
10.1007/s00421-012-2531-5
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发表时间：
2013
影响因子：
3
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Masaki Takahashi;et al.
本研究的目的是调查低容量运动训练（100分钟/周）对老年人氧化应激和中性粒细胞活化标志物的影响。28名老年人（年龄范围65 - 78岁）被分配到对照组（n = 14）或运动组（n = 14）。运动计划包括步行30 - 60分钟/次，每周2天，持续12周。在开始治疗前（基线）和治疗结束时采集血样。使用商业试剂盒测量空腹血浆和血清氧化应激和炎症标志物。使用流式细胞术测定循环白细胞上粘附分子（CD66 b和CD62 L）的细胞表面表达。活性氧代谢物衍生物浓度仅在运动组有低于基线值的趋势（P = 0.05）。生物抗氧化能力、硫氧还蛋白浓度和谷胱甘肽过氧化物酶活性仅在运动组显著升高（均P <0.05）。运动组CD66 b表达呈下降趋势，而CD62 L表达则显著升高（P <0.05）。我们的研究结果表明，运动训练低于目前推荐的水平，至少150分钟/周减弱基础氧化应激和中性粒细胞活化的老年人。因此，我们的研究结果可能会鼓励更多的人将少量的体育活动纳入他们的生活。
The purpose of this study was to investigate the effects of low-volume exercise training (100 min/week) on oxidative stress and neutrophils activation markers in older adults. Twenty-eight older adults (age range 65–78 years) were assigned into control (n= 14) or exercise (n= 14) groups. The exercise program consisted of walking 30–60 min/session, 2 days each week for 12 weeks. Blood samples were taken before starting the sessions (baseline) and when they ended. Fasting plasma and serum oxidative stress and inflammatory markers were measured using commercial kits. Cell surface expression of adhesion molecules on circulating leukocytes (CD66b and CD62L) was determined using flow cytometry. The concentrations of derivatives of reactive oxygen metabolites tended to be lower than the baseline values only in the exercise group (P= 0.05). The biological antioxidant potential, thioredoxin concentrations, and glutathione peroxidase activities significantly increased only in the exercise group (P< 0.05 for all). While CD66b expression tended to decrease only in the exercise group, CD62L expression significantly increased (P< 0.05). Our findings indicate that exercise training below the current recommended level of at least 150 min/week attenuates basal oxidative stress and neutrophil activation in older adults. Thus, our findings may encourage more people to incorporate a small amount of physical activity into their lives.