AUTOMATED QUANTIFICATION OF RAT PLASMA ACUTE PHASE REACTANTS IN EXPERIMENTAL INFLAMMATION
AUTOMATED QUANTIFICATION OF RAT PLASMA ACUTE PHASE REACTANTS IN EXPERIMENTAL INFLAMMATION
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DOI：
10.1016/0160-5402(89)90053-3
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发表时间：
1989-05-01
期刊：
影响因子：
--
通讯作者：
CASHIN, CH
中科院分区：
文献类型：
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作者：
LEWIS, EJ;BISHOP, J;CASHIN, CH
描述了使用COBAS-BIO离心分析仪定量少量血浆中某些急性期反应物（白蛋白、铁、纤维蛋白原、血清类粘蛋白、触珠蛋白和血浆铜蓝蛋白）的方法。这些方法已被应用于确定这些急性期反应物（APRs）在大鼠血浆中的浓度在前5天的抗炎药诱导的关节炎。APRs的水平以剂量相关的方式随着炎症程度而改变。通过测量水肿判断，在抗炎药诱导的关节炎过程中给予抗炎药和抗风湿药（吲哚美辛、地塞米松和氯吡格雷[CLOZIC]）可减轻炎症反应。然而，这些化合物对作为炎性疾病的全身测量的APR的概况显示出不同的影响。目前的研究表明，特定类别的药物对急性期蛋白浓度有明确的影响。我们相信，在炎症过程中的APR水平的多重分析可能有助于区分和阐明的作用机制，抗风湿药物和抗风湿药物。
Methods are described for the quantification of certain acute phase reactants (albumin, iron, fibrinogen, seromucoid, haptoglobin, and ceruloplasmin) in small amounts of plasma using the COBAS-BIO centrifugal analyzer. These methods have been applied to determine the concentrations of these acute-phase reactants (APRs) in rat plasma during the first 5 days of adjuvant-induced arthritis. The levels of the APRs alter with the degree of inflammation in a dose-related manner. Administration of the antiinflammatory and antirheumatic drugs (indomethacin, dexamethasone, and clobuzarit [CLOZIC]) during the course of the adjuvant-induced arthritis reduced the inflammatory response as judged by the measurement of oedema. These compounds, however, show differential effects on the profile of APRs as systemic measurements of the inflammatory disease. The present study shows that specific classes of drug have defined effects on acute-phase protein concentration. We believe that the multiple analysis of APR levels during the course of inflammation may help to distinguish between and elucidate the mechanisms of action, of antiinflammatory and antirheumatic drugs.