Overexpression of AEG-1 in renal cell carcinoma and its correlation with tumor nuclear grade and progression

Overexpression of AEG-1 in renal cell carcinoma and its correlation with tumor nuclear grade and progression
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肾细胞癌中 AEG-1 的过度表达及其与肿瘤核分级和进展的相关性。

DOI：
10.4149/neo_2010_06_522
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发表时间：
2010-01-01
期刊：
NEOPLASMA
影响因子：
3
通讯作者：
Wang, L.
Wang, L.
中科院分区：
医学4区
文献类型：
--
作者：
Chen, W.;Ke, Z.;Wang, L.

文献摘要

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未标记 本研究旨在检测一种新发现的癌基因--星形胶质细胞升高基因-1（AEG-1）在肾细胞癌（RCC）中的表达及其与病理组织学特征和患者生存期的关系。实时荧光定量RT-PCR和Western blot检测结果显示，AEG-1在8例肾癌组织中的表达明显高于同一患者的配对正常组织。AEG-1的表达水平在四个RCC细胞系与正常肾小管上皮人肾细胞2（HK-2）相比，在mRNA和蛋白质水平也增加。此外，免疫组化分析显示AEG-1在102例（94.1%）石蜡包埋肾细胞癌组织中高表达。AEG-1的表达与肿瘤分级（P <0.001）、临床分期（P = 0.003）、T分期（P = 0.003）和转移分期（P=0.032）相关。AEG-1低表达组平均生存时间为76.98 m，高表达组平均生存时间为60.94 m。我们的研究结果表明，AEG-1蛋白在RCC中过表达，并在肿瘤的分化和进展中发挥重要作用。AEG-1高表达与预后不良密切相关。 关键词 星形胶质细胞升高基因-1，AEG-1，肾癌，预后。
UNLABELLED The study was aimed at detecting the expression of a newly found oncogene, astrocyte elevated gene-1 (AEG-1), in renal cell carcinoma (RCC) and its correlation with histopathologic features and the survival of patients. Real-time reverse transcription-PCR and Western blot showed markedly higher expression of AEG-1 in 8 cases of RCC tissue compared with the paired normal tissue from the same patient. The expression level of AEG-1 was also increased in four RCC cell lines in contrast with normal tubular epithelial human kidney cells 2 (HK-2) at both mRNA and protein levels. Furthermore, immunohistochemistry analysis showed highly expressed AEG-1 in 96 of 102 (94.1%) cases of paraffin-embedded archival RCC tissue. Statistical analysis showed a significant correlation of AEG-1 expression with tumor grade (P <0.001), clinical staging (P = 0.003), T classification (P = 0.003) as well as metastasis classification (P=0.032). The means for survival time of low AEG-1 expression group was 76.98mwhile high AEG-1expression group was 60.94m. Our results suggest that AEG-1 protein is overexpressed in RCC and plays an important role in tumor differentiation and progression. High AEG-1 expression is closely associated with poor prognosis. KEYWORDS Astrocyte elevated gene-1, AEG-1, renal cell carcinoma, prognosis.