Red Light-Aphototropic (rap) Mutants Lack Red Light-Induced Chloroplast Relocation Movement in the Fern Adiantum capillus-veneris
Red Light-Aphototropic (rap) Mutants Lack Red Light-Induced Chloroplast Relocation Movement in the Fern Adiantum capillus-veneris
复制标题
铁线蕨中红光抗光性（rap）突变体缺乏红光诱导的叶绿体迁移运动
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
M. Wada
中科院分区：
文献类型：
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作者：
A. Kadota;M. Wada
两类生理上不同的光敏色素似乎调节着蕨类植物单相中许多不同的红光刺激反应。这些类别包括二色性光敏色素和非二色性光敏色素[Kadota等人。(1989)植物细胞生理学。30：523]。前者调控的反应在偏振光下表现为行为二向色性，而后者调控的反应则不表现为行为二色性。二向色性光敏色素在向光性和叶绿体定位反应中的作用尤为明显。在本工作中，我们分离了蕨类植物铁线蕨红光趋光性(RAP)突变体，并研究了它们的光响应。共获得了5个Rap突变体：Rap2、Rap7、Rap32、Rap33、Rap39。RAP突变体缺乏红光偏光性，但仍保持正常的蓝光偏光性和偏光性。光敏色素发色团的前体胆绿素对RAP突变体的红光趋光性没有影响。对其他光反应的分析表明，突变体缺少光敏色素介导的叶绿体定位运动，而蓝光诱导的反应是正常的。在RAP菌株中，光敏色素对孢子萌发、顶端生长和细胞分裂的调节也是正常的。这些结果表明，向光性和叶绿体定位运动具有相同的二色性光敏色素和以下信号转导步骤。此外，这一途径不同于控制孢子萌发、顶端生长和细胞分裂的途径。RAP突变体可能在光感受器分子本身、与稳定结构的结合机制或参与二色性光敏色素介导的信号转导途径的早期元件上存在缺陷(S)。
Two physiologically distinct classes of phytochrome seem to regulate the many diverse red light-stimulated responses in the fern haplophase. The classes include dichroic phytochrome and non-dichroic phytochrome [Kadota et al. (1989) Plant CellPhysiol. 30: 523]. The responses regulated by the former show action dichroism under polarized light while those regulated by the latter do not. The involvement of dichroic phytochrome is particularly evident in phototropism and the chloroplast relocation response. In the present work, red-light aphototropic (rap) mutants of the fern Adiantum capillus-veneris were isolated and their photoresponses were characterized. Five rap mutants, rap2, rap7, rap32, rap33, rap39 were obtained. The rap mutants lacked red light polarotropism but still retained normal blue light phototropism and polarotropism. Treatment with biliverdin, a precursor of phytochrome chromophore had no effect on the red-light aphototropic nature of rap mutants. Analyses on the other photoresponses revealed that phytochrome-mediated chloroplast relocation movement was missing in the mutants whereas the response induced by blue light was normal. Phytochrome regulation of spore germination, tip growth and cell division were also normal in the rap strains. These results indicate that phototropism and chloroplast relocation movement share the same dichroic phytochrome and the following signal transduction steps. Moreover, it shows that this pathway is distinct from that controlling spore germination, tip growth and cell division. The rap mutants may have defect(s) in the photoreceptor molecule itself, in the binding mechanism to a stable structure, or in elements involved early in the dichroic phytochrome-mediated signal transduction pathway.
影响因子：
11.6
作者：
LISCUM, E;BRIGGS, WR
通讯作者：
BRIGGS, WR