INHIBITOR ACTION OF THE ANTIMALARIAL COMPOUND ATOVAQUONE (566C80) AGAINST PLASMODIUM-BERGHEI ANKA IN THE MOSQUITO, ANOPHELES-STEPHENSI
INHIBITOR ACTION OF THE ANTIMALARIAL COMPOUND ATOVAQUONE (566C80) AGAINST PLASMODIUM-BERGHEI ANKA IN THE MOSQUITO, ANOPHELES-STEPHENSI
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DOI：
10.1017/s0031182000075922
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发表时间：
1994-05-01
期刊：
影响因子：
2.4
通讯作者：
SINDEN, RE
中科院分区：
文献类型：
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作者：
FOWLER, RE;BILLINGSLEY, PF;SINDEN, RE
阿托伐醌抗伯氏疟原虫ANKA的活性在孢子发育过程中进行了检查。斯氏按蚊以配子体感染的小鼠喂养，这些小鼠先前已用阿托伐醌或稀释剂单独处理8小时。苔藓虫中肠进行了检查卵囊，唾液腺感染估计使用ELISA环子孢子蛋白（CSP）。当以0.1-10 mg阿托伐醌/kg体重给药的小鼠喂食蚊子时，每个中肠的卵囊数量至少下降97%。受卵囊感染的蚊子的流行率从对照组的70-90%分别下降到40%或10%。在100 mg/kg剂量下未观察到卵囊。CSP ELISA结果表明，阿托伐醌喂养的蚊子不能产生子孢子。以配子母细胞血症、阿托伐醌处理的小鼠（0.1-100 mg/kg）为食的蚊子不会将疟疾传播给未接触过的小鼠。这些结果表明，阿托伐醌对伯氏疟原虫的蚊子阶段具有高度有效的抑制活性。
The activity of atovaquone against Plasmodium berghei ANKA during sporogonic development has been examined. Anopheles stephensi mosquitoes were fed on gametocyte infected mice which had been treated 8h previously with atovaquone or diluent alone. Mosquito midguts were examined for oocysts, and salivary gland infections were estimated using an ELISA for the circumsporozoite protein (CSP). The number of oocysts per midgut fell by at least 97% when mosquitoes were fed on mice dosed with 0.1-10 mg atovaquone/kg body weight. This was paralleled by a decrease in the prevalence of oocyst-infected mosquitoes from 70-90% in controls to 40% or 10% respectively. No oocysts were observed at a dose of 100 mg/kg. CSP ELISA results indicated that mosquitoes fed on atovaquone failed to produce sporozoites. Mosquitoes which fed on gametocytaemic, atovaquone-treated mice (0.1-100 mg/kg) did not transmit malaria to naive mice. These results demonstrate that atovaquone has a highly potent inhibitory activity against the mosquito stages of P. berghei.