Stimulation of Lipolysis in Humans by Physiological Hypercortisolemia
Stimulation of Lipolysis in Humans by Physiological Hypercortisolemia
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DOI：
10.2337/diab.40.10.1228
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发表时间：
1991-10
期刊：
影响因子：
7.7
通讯作者：
G. Divertie;M. Jensen;J. Miles
中科院分区：
文献类型：
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作者：
G. Divertie;M. Jensen;J. Miles
糖皮质激素对动物和人类脂肪组织脂解作用的影响存在争议。为了确定血浆皮质醇的生理性增加（类似于糖尿病酮症酸中毒和其他应激条件下观察到的情况）是否会刺激脂肪分解，以[1-14C]棕榈酸酯作为示踪剂，对七名非糖尿病志愿者进行了两次棕榈酸酯动力学测量。受试者随机接受 2 μg·kg−1·min−1 氢化可的松或生理盐水输注 6 小时。在这两种情况下，均使用胰钳（0.12 μg·kg−1·min−1 生长抑素、0.05 mU·kg−1·min−1 胰岛素和 3 ng·kg−1·min−1 生长激素）将血浆激素浓度维持在所需水平。在皮质醇输注过程中，血浆皮质醇浓度增加至~970 nM。在皮质醇输注期间，棕榈酸酯的出现率 (Ra) 和浓度增加了约 60%，但在盐水输注期间没有变化。通过曲线下面积分析比较，在 6 小时的研究中，皮质醇 Ra 和浓度的增量显着大于盐水输注（分别为 152 ± 52 与 -48 ± 23 μmol·kg−1 和 12.2 ± 4.1 与 -4.9 ± 4.1 mmol·min−1·L−1；P < 0.02）。皮质醇（5.0 ± 0.3 vs. 6.1 ± 0.6 mM，NS）或盐水（4.9 ± 0.2 vs. 4.9 ± 0.1 mM，NS）输注期间血浆葡萄糖浓度没有显着变化。在非糖尿病志愿者中，输注 6 小时皮质醇可导致棕榈酸酯 Ra 增加 60%，而输注盐水则不会出现这种情况。因此，生理性高皮质醇血症可能导致人类在压力期间观察到的脂肪分解速率增加。
The effect of glucocorticoids on adipose tissue lipolysis in animals and humans is controversial. To determine whether a physiological increase in plasma cortisol, similar to that observed in diabetic ketoacidosis and other stress conditions, stimulates lipolysis, palmitate kinetics were measured in seven nondiabetic volunteers on two occasions with [1-14C]palmitate as a tracer. Subjects received a 6-h infusion of either 2 μg · kg−1 · min−1 hydrocortisone or saline in random order. On both occasions, a pancreatic clamp (0.12 μg · kg−1 · min−1 somatostatin, 0.05 mU · kg−1 · min−1 insulin, and 3 ng · kg−1 · min−1 growth hormone) was used to maintain plasma hormone concentrations at desired levels. Plasma cortisol concentrations increased to ∼970 nM during cortisol infusion. Palmitate rate of appearance (Ra) and concentration increased by ∼60% during cortisol infusion but did not change during saline infusion. Increments in palmitate Ra and concentration over the 6-h study were significantly greater during cortisol than saline infusion when compared by area-under-the-curve analysis (152 ± 52 vs. −48 ± 23 μmol · kg−1 and 12.2 ± 4.1 vs. −4.9 ± 4.1 mmol · min−1 · L−1, respectively; P < 0.02). Plasma glucose concentrations did not change significantly during cortisol (5.0 ± 0.3 vs. 6.1 ± 0.6 mM, NS) or saline (4.9 ± 0.2 vs. 4.9 ± 0.1 mM, NS) infusion. In nondiabetic volunteers, a 6-h cortisol infusion was associated with a 60% increase in palmitate Ra that did not occur with saline infusion. Thus, physiological hypercortisolemia may contribute to the increased rates of lipolysis observed in humans during stress.