INHIBITION OF THE GLYCOLYTIC PATHWAY BY METHYLGLYOXAL IN HUMAN-PLATELETS
INHIBITION OF THE GLYCOLYTIC PATHWAY BY METHYLGLYOXAL IN HUMAN-PLATELETS
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DOI：
10.1002/cbf.290070111
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发表时间：
1989-01-01
影响因子：
3.6
通讯作者：
BUZZI, E
中科院分区：
文献类型：
--
作者：
LEONCINI, G;MARESCA, M;BUZZI, E
将人血小板与甲基乙二醛和葡萄糖一起孵育，通过谷胱甘肽酶系统将酮醛快速转化为D-乳酸，并使GSH部分还原。葡萄糖利用在糖酵解途径水平受到影响。通过磷酸己糖分流，酮醛对糖原分解和葡萄糖氧化没有影响。磷酸果糖激酶、果糖1，6二磷酸（F1，6DP）醛缩酶、3-磷酸甘油醛脱氢酶和3-磷酸甘油酸脱氢酶主要受丙酮醛抑制。观察到乳酸和丙酮酸形成减少以及一些糖酵解中间体（果糖1，6二磷酸、二羟丙酮磷酸、3-磷酸甘油酸）的积累。此外，丙酮醛诱导代谢ATP浓度下降。由于丙酮醛是糖酵解旁路酶系统从磷酸二羟丙酮到D-乳酸的中间产物，因此可以认为酮醛对丙糖代谢有调节作用。
The incubation of human platelets with methylglyoxal and glucose produces a rapid transformation of the ketoaldehyde to D-lactate by the glyoxalase system and a partial reduction in GSH. Glucose utilization is affected at the level of the glycoltytic pathway. No effect of the ketoaldehyde on glycogenolysis and glucose oxidation through the hexose monophosphate shunt was demonstrated. Phosphofructokinase, fructose 1,6 diphosphate (F1, 6DP) aldolase, glyceraldehyde 3-phosphate dehydrogenase and 3-phosphoglycerate mutase wree mostly inhibited by methylglyoxal. A decrease in lactate and pyruvate formation and an accumulation of some glycolytic intermediates (fructose 1,6 diphosphate, dihydroxyacetone phosphate, 3-phosphoglycerate) was observed. Moreover methylglyoxal induced a fall in the metabolic ATP concentration. Since methlglyoxal is an intermediate of the glycolytic bypase system form dihydroxyacetone phosphate to D-lactate, it may be assumed that ketoaldehyde exerts a rgulating effect on triose metabolism.