Transbilayer movement of phospholipids at the main phase transition of lipid membranes:: Implications for rapid flip-flop in biological membranes
Transbilayer movement of phospholipids at the main phase transition of lipid membranes:: Implications for rapid flip-flop in biological membranes
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DOI：
10.1016/s0006-3495(02)75332-0
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发表时间：
2002-12-01
影响因子：
3.4
通讯作者：
Müller, P
中科院分区：
文献类型：
--
作者：
John, K;Schreiber, S;Müller, P
本文研究了荧光磷脂类似物在磷脂膜发生脂相转变时在脂质体中的跨双层运动。使用了两种NBD标记的类似物，一种在sn-2位的短脂肪酸链上带有荧光部分（C-6 NBD-PC），一种具有两个长脂肪酰基链的头基标记的类似物（N-NBD-PE）。通过基于连二亚硫酸盐的测定评估类似物的跨双层再分布。在DPPC（T-c = 41 ℃）和DMPC（T-c = 23 ℃）的脂相转变时，我们观察到这两种类似物的跨双层运动急剧增加。在DPPC的T-c处，类似物的翻转快，半衰期（t（1/2））接近6 -10 min，在DMPC的T-c处，类似物的翻转更快，t（1/2）约为
The transbilayer movement of fluorescent phospholipid analogs in liposomes was studied at the lipid phase transition of phospholipid membranes. Two NBD-labeled analogs were used, one bearing the fluorescent moiety at a short fatty acid chain in the sn-2 position (C-6 NBD-PC) and one headgroup-labeled analog having two long fatty acyl chains (N-NBD-PE). The transbilayer redistribution of the analogs was assessed by a dithionite-based assay. We observed a drastic increase of the transbilayer movement of both analogs at the lipid phase transition of DPPC (T-c = 41degreesC) and DMPC (T-c = 23degreesC). The flip-flop of analogs was fast at the T-c of DPPC with a half-time (t(1/2)) of similar to6-10 min and even faster at the T-c of DMPC with t(1/2) on the order of