"Slow Activity Transients" in Infant Rat Visual Cortex: A Spreading Synchronous Oscillation Patterned by Retinal Waves
"Slow Activity Transients" in Infant Rat Visual Cortex: A Spreading Synchronous Oscillation Patterned by Retinal Waves
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DOI：
10.1523/jneurosci.4995-09.2010
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发表时间：
2010-03-24
影响因子：
5.3
通讯作者：
Khazipov, Rustem
中科院分区：
文献类型：
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作者：
Colonnese, Matthew T.;Khazipov, Rustem
早产儿脑电图的一个主要特征是存在包含称为慢活动瞬变（SAT）的快速振荡的大亚慢电位。这种活性尚未在动物模型中描述，其生成机制也未知。在这里，我们使用直流和多位点的细胞外，以及全细胞，在体内记录，以证明存在定期重复SAT在婴幼儿大鼠的视觉皮层睁眼前。出生后第10-11天的SAT仅在无麻醉的情况下出现，可识别为一组单独的长持续时间（类似于10 s）事件，由快速振荡活动（15-30 Hz）的多次爆发总和产生的大（>1 mV）负局部场电位组成。在睁眼之前，SAT使视觉皮层中的绝大多数神经元活动（87%）同步。眼球摘除消除了SAT，其持续时间，间隔时间，和子突发结构相匹配的III相视网膜波记录在体外。然而，视网膜波缺乏快速振荡，这表明它们起源于中央。多电极记录显示，SAT在皮层水平分布，并通过快速振荡同步活动在共同活动的区域。SAT明显不同于正在进行的皮质活动，这是可观察到的，作为一个单独的类从出生后第9天的短爆发。总之，我们的数据表明，在体内，早期皮层活动主要是由周边输入-视网膜波在视觉皮层-提供兴奋性输入，并通过丘脑皮层电路，它将这种输入转换为β振荡。我们认为SAT的同步振荡参与了视觉回路的形成。
A primary feature of the preterm infant electroencephalogram is the presence of large infra-slow potentials containing rapid oscillations called slow activity transients (SATs). Such activity has not been described in animal models, and their generative mechanisms are unknown. Here we use direct-current and multisite extracellular, as well as whole-cell, recording in vivo to demonstrate the existence of regularly repeating SATs in the visual cortex of infant rats before eye opening. Present only in absence of anesthesia, SATs at postnatal day 10-11 were identifiable as a separate group of long-duration (similar to 10 s) events that consisted of large (>1 mV) negative local-field potentials produced by the summation of multiple bursts of rapid oscillatory activity (15-30 Hz). SATs synchronized the vast majority of neuronal activity (87%) in the visual cortex before eye opening. Enucleation eliminated SATs, and their duration, interevent interval, and sub-burst structure matched those of phase III retinal waves recorded in vitro. Retinal waves, however, lacked rapid oscillations, suggesting that they arise centrally. Multielectrode recordings showed that SATs spread horizontally in cortex and synchronize activity at coactive locales via the rapid oscillations. SATs were clearly different from ongoing cortical activity, which was observable as a separate class of short bursts from postnatal day 9. Together, our data suggest that, in vivo, early cortical activity is primarily determined by peripheral inputs-retinal waves in visual cortex-that provide excitatory input, and by thalamocortical circuitry, which transforms this input to beta oscillations. We propose that the synchronous oscillations of SATs participate in the formation of visual circuitry.