Silver nanoparticles as antimicrobial agent:: a case study on E-coli as a model for Gram-negative bacteria
Silver nanoparticles as antimicrobial agent:: a case study on E-coli as a model for Gram-negative bacteria
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DOI：
10.1016/j.jcis.2004.02.012
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发表时间：
2004-07-01
影响因子：
9.9
通讯作者：
Salopek-Sondi, B
中科院分区：
文献类型：
--
作者：
Sondi, I;Salopek-Sondi, B
银纳米颗粒对大肠杆菌的抗菌活性被研究为革兰氏阴性细菌的模型。在固体琼脂板上的Luria-Bertani（LB）培养基和补充有不同浓度的纳米化银颗粒的液体系统中进行了细菌学测试。这些颗粒被证明是有效的杀菌剂。扫描和透射电子显微镜（SEM和TEM）用于研究这种纳米级材料的杀生物作用。结果证实，处理过的大肠杆菌细胞受损，显示细菌细胞壁中“凹坑”的形成，而发现银纳米颗粒会积聚在细菌膜中。具有这种形态的膜表现出渗透性的显着增加，导致细胞死亡。这些无毒的纳米材料可以以简单且具有成本效益的方式制备，可能适合制定新型的杀菌材料。 （c）2004 Elsevier Inc.保留所有权利。
The antimicrobial activity of silver nanoparticles against E. coli was investigated as a model for Gram-negative bacteria. Bacteriological tests were performed in Luria-Bertani (LB) medium on solid agar plates and in liquid systems supplemented with different concentrations of nanosized silver particles. These particles were shown to be an effective bactericide. Scanning and transmission electron microscopy (SEM and TEM) were used to study the biocidal action of this nanoscale material. The results confirmed that the treated E. coli cells were damaged, showing formation of "pits" in the cell wall of the bacteria, while the silver nanoparticles were found to accumulate in the bacterial membrane. A membrane with such a morphology exhibits a significant increase in permeability, resulting in death of the cell. These nontoxic nanomaterials, which can be prepared in a simple and cost-effective manner, may be suitable for the formulation of new types of bactericidal materials. (C) 2004 Elsevier Inc. All rights reserved.