Illumination with blue light reactivates respiratory activity of mitochondria inhibited by nitric oxide, but not by glycerol trinitrate
Illumination with blue light reactivates respiratory activity of mitochondria inhibited by nitric oxide, but not by glycerol trinitrate
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DOI：
10.1016/j.abb.2008.01.009
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发表时间：
2008-03-15
影响因子：
3.9
通讯作者：
Kozlov, Andrey V.
中科院分区：
文献类型：
--
作者：
Dungel, Peter;Mittermayr, Rainer;Kozlov, Andrey V.
已知一氧化氮（NO）可逆地抑制线粒体呼吸。本研究旨在阐明特定波长的低水平照明是否恢复线粒体呼吸抑制NO和甘油三硝酸酯（GTN），临床上使用的NO模拟。在感染性休克的浓度下，NO完全抑制肝线粒体的呼吸。在波长为430 nm的光照下，呼吸完全恢复，而较长的波长则不太有效。GTN抑制线粒体呼吸，虽然GTN的效率低于在脓毒症模型中观察到的NO浓度。然而，GTN抑制是绝对不敏感的照明，无论使用的波长。我们的数据表明，可见光的短波长有效地促进恢复的线粒体抑制NO-气体的水平下产生的败血症条件下。GTN对线粒体呼吸的抑制作用对可见光不敏感，提示其抑制机制不同于NO介导。(C)2008年爱思唯尔公司All rights reserved.
Nitric oxide (NO) is known to inhibit mitochondrial respiration reversibly. This study aimed at clarifying whether low level illumination at specific wavelengths recovers mitochondrial respiration inhibited by NO and glycerol-trinitrate (GTN), a clinically used NO mimetic. NO fully inhibited respiration of liver mitochondria at concentrations occurring under septic shock. The respiration was completely restored by illumination at the wavelength of 430 nm while longer wavelengths were less effective. GTN inhibited mitochondrial respiration though the efficiency of GTN was lower compared to NO concentrations observed in sepsis models. However, GTN inhibition was absolutely insensitive to illumination regardless of wavelength used. Our data show that visible light of short wavelengths efficiently facilitates the recovery of mitochondria inhibited by NO-gas at the levels generated under septic conditions. The inhibition of mitochondrial respiration by GTN is not sensitive to visible light, suggesting an inhibition mechanism other that NO mediation. (C) 2008 Elsevier Inc. All rights reserved.