Role of arachidonic acid metabolism in the mitogenic response of BALB/c 3T3 fibroblasts to epidermal growth factor.
Role of arachidonic acid metabolism in the mitogenic response of BALB/c 3T3 fibroblasts to epidermal growth factor.
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花生四烯酸代谢在 BALB/c 3T3 成纤维细胞对表皮生长因子的有丝分裂反应中的作用。
DOI：
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发表时间：
1988
影响因子：
3.6
通讯作者：
Thomas E. Eling
中科院分区：
文献类型：
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作者：
R. D. Nolan;R. Danilowicz;Thomas E. Eling
我们已经研究了花生四烯酸释放和代谢在促有丝分裂反应中的参与，即，[~ 3 H]胸腺嘧啶核苷掺入法，对BALB/c 3 T3细胞表皮生长因子（EGF）的影响。EGF以刺激有丝分裂的相同浓度加入BALB/c 3 T3细胞后，诱导花生四烯酸和前列腺素（PG）形成的释放。此外，EGF刺激的有丝分裂被花生四烯酸代谢的抑制剂阻断，包括吲哚美辛、二十碳四炔酸和地塞米松，而在BALB/c 3 T3细胞中添加主要花生四烯酸产物PGE 2、PGF 2 α及其中间体PGG 2和PGH 2与EGF协同刺激有丝分裂。此外，PGs的BALB/c 3 T3细胞也克服了吲哚美辛和二十碳四炔酸抑制EGF的反应。吲哚美辛必须与EGF一起加入，以阻断花生四烯酸代谢和随后的有丝分裂。这些结果表明，花生四烯酸的释放及其随后的代谢是EGF启动细胞周期穿越的明显早期要求。
We have investigated the involvement of arachidonic acid release and metabolism in the mitogenic response, i.e., [3H]thymidine incorporation, to epidermal growth factor (EGF) in BALB/c 3T3 cells. EGF induces release of arachidonate and prostaglandin (PG) formation after its addition to BALB/c 3T3 cells at the same concentrations that stimulate mitogenesis. Further, EGF-stimulated mitogenesis is blocked by inhibitors of arachidonate metabolism including indomethacin, eicosatetraynoic acid, and dexamethasone, whereas the addition of major arachidonate products in BALB/c 3T3 cells, PGE2, PGF2 alpha, and their intermediates PGG2 and PGH2, stimulate mitogenesis in synergism with EGF. The addition of PGs to BALB/c 3T3 cells also overcame indomethacin- and eicosatetraynoic acid-inhibited responses to EGF. Indomethacin must be added with EGF in order to block arachidonate metabolism and subsequent mitogenesis. These results suggest that the release of arachidonic acid and its subsequent metabolism is an apparent early requirement for the initiation of cell cycle traversal by EGF.