TRANSPLANTATION OF PURIFIED POPULATIONS OF SCHWANN-CELLS INTO LESIONED ADULT-RAT SPINAL-CORD
TRANSPLANTATION OF PURIFIED POPULATIONS OF SCHWANN-CELLS INTO LESIONED ADULT-RAT SPINAL-CORD
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DOI：
10.1007/bf00939240
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发表时间：
1994-12-01
影响因子：
6
通讯作者：
BUNGE, MB
中科院分区：
文献类型：
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作者：
BUNGE, MB
周围神经和纯化的雪旺细胞群都能促进周围和中枢神经系统的轴突再生。为了评估雪旺细胞是否能够提供一座桥梁，使成年脊髓损伤区域的下行和上升轴突得以再生，将包裹在胶原卷轴中的雪旺细胞移植到通过光化学方法产生的病变中。植入后28~90天可见大量有髓和无髓轴突，雪旺细胞正常有髓或包裹入内轴突。相比之下，脱细胞胶原移植物不含轴突。因此，雪旺细胞刺激大量轴突生长到移植物中。部分是为了解决致密的胶原层作为屏障的担忧，我们评估了在T8时切除T9-11节段的成年近交系大鼠脊髓横断后，雪旺细胞在半透性聚丙烯腈/聚氯乙烯(PAN/PVC)引导通道内的移植。移植1个月后，雪旺细胞种植通道中的有髓和无髓轴突比对照组多。脊髓上轴突没有侵入通道；一些是外周起源的，另一些是脊髓中间神经元，发现距离移植物长达9个节段。当脊髓的两端被插入充满雪旺细胞的通道时，一条带血管的组织缆桥接脊髓的两端，其中包含大量的有髓轴突和更多的来自脊髓灰质和背根神经节神经元的无髓轴突。
Both peripheral nerve and purified populations of Schwann cells promote axonal regeneration in the peripheral and central nervous systems. In order to assess whether Schwann cells can provide a bridge enabling regrowth of descending and ascending axons across an area of injury in adult spinal cord, Schwann cells enclosed within a collagen scroll were transplanted into lesions created photochemically. Numerous myelinated and unmyelinated axons were found throughout 28-90 day implants; Schwann cells myelinated or ensheathed the ingrowing axons normally. In contrast, acellular collagen grafts did not contain axons. Thus, Schwann cells stimulated abundant growth of axons into the grafts. In part to address the concern that the dense collagen layer acted as a barrier, we assessed transplantation of Schwann cells, inside semipermeable polyacrylonitrile/polyvinylchloride (PAN/PVC) guidance channels, after transection of adult inbred rat spinal cords at T8 with removal of the the T9-11 segments. One month after grafting, a vascularized tissue cable was present with more myelinated and unmyelinated axons in the Schwann cell seeded channels than controls. Supraspinal axons did not invade the channel; some were of peripheral origin and others were spinal cord interneurons found up to nine segments away from the graft. When both cut ends of the cord were from inserted into Schwann cell filled channels, a vascularized tissue cable bridged the ends of the spinal cord, containing numerous myelinated axons and more unmyelinated axons, originating from spinal grey matter and dorsal root ganglion neurons.