The effect of acute alcohol intoxication on gut wall integrity in healthy male volunteers; a randomized controlled trial
The effect of acute alcohol intoxication on gut wall integrity in healthy male volunteers; a randomized controlled trial
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DOI：
10.1016/j.alcohol.2014.09.033
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发表时间：
2015-02-01
期刊：
影响因子：
2.3
通讯作者：
Hulscher, J. B.
中科院分区：
文献类型：
--
作者：
de Jong, W. J.;Cleveringa, A. M.;Hulscher, J. B.
本研究的目的是确定急性饮酒对肠细胞的影响。已知长期饮酒会导致主动饮酒的酗酒者和酒精性肝病患者的肠壁完整性降低。关于急性饮酒对健康人造成的损害程度的数据很少。研究表明，在现代社会，大量的偶然饮酒是一个日益严重的问题。关于这一问题的数据可能会为健康个体提供这种行为后果的见解。在一项交叉设计的随机临床试验中，15名健康志愿者每天饮用水，另一天饮用酒精。在食用前采集一份血液样本，食用后每小时采集五份，24小时后采集一份。肠脂肪酸结合蛋白（I-FABP）被用作肠上皮细胞损伤的标志物。肝脂肪酸结合蛋白（L-FABP）、天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）和γ-谷氨酰转移酶（GGT）被用作肝细胞损伤的标志物。脂多糖结合蛋白（LBP）和可溶性CD 14（sCD 14）被用来作为衡量易位。白细胞介素-6（IL-6）用于评估对内毒素血症的急性炎症反应。与饮水量相比，饮酒导致血清I-和L-FABP水平显著增加。水平直接增加消费后，并在4小时内下降到正常水平。LBP、sCD 14和IL-6水平在酒精组中没有显著升高。中度急性饮酒会立即损害肠上皮细胞，但似乎不会引起内毒素血症。(C)2015 Elsevier Inc. All rights reserved.
The aim of the study is to determine the effect of acute alcohol consumption on enterocytes. Chronic alcohol consumption has been known to induce a decrease in gut wall integrity in actively drinking alcoholics and patients with alcohol-induced liver disease. Data on the extent of the damage induced by acute alcohol consumption in healthy human beings is scarce. Studies show that heavy incidental alcohol consumption is a growing problem in modern society. Data on this matter may provide insights into the consequences of this behavior for healthy individuals. In a randomized clinical trial in crossover design, 15 healthy volunteers consumed water one day and alcohol the other. One blood sample was collected pre-consumption, five every hour post-consumption, and one after 24 h. Intestinal fatty acid binding protein (I-FABP) was used as a marker for enterocyte damage. Liver fatty acid binding protein (L-FABP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyl transferase (GGT) were used as markers for hepatocyte damage. Lipopolysaccharide binding protein (LBP) and soluble CD14 (sCD14) were used as a measure of translocation. Interleukin-6 (IL-6) was used to assess the acute inflammatory response to endotoxemia. Alcohol consumption caused a significant increase in serum I- and L-FABP levels, compared to water consumption. Levels increased directly post-consumption and decreased to normal levels within 4 h. LBP, sCD14, and IL-6 levels were not significantly higher in the alcohol group. Moderate acute alcohol consumption immediately damages the enterocyte but does not seem to cause endotoxemia. (C) 2015 Elsevier Inc. All rights reserved.