Fluorinated alpha-methylamino acids as 19F NMR indicators of intracellular pH.
Fluorinated alpha-methylamino acids as 19F NMR indicators of intracellular pH.
复制标题
氟化 α-甲基氨基酸作为细胞内 pH 值的 19F NMR 指示剂。
DOI：
10.1016/s0006-3495(83)84349-5
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发表时间：
1983
影响因子：
3.4
通讯作者：
Deutsch,C
中科院分区：
文献类型：
--
作者：
Taylor,JS;Deutsch,C
用19F核磁共振法测定了一系列无毒的氟化氨基酸及其甲酯在人外周血淋巴细胞内的pH值。对单氟、二氟和三氟α -甲基丙氨酸及其甲酯的光谱、氟共振的pH依赖性以及温度和阳离子对NH2pKa值的影响进行了表征。这些pH指标可以单独使用或组合使用，以确定细胞外pH值范围（大于2个pH单位）的细胞内与细胞外pH值。
A series of nontoxic, fluorinated amino acids and their methyl esters have been used to measure intracellular pH in human peripheral blood lymphocytes by 19F NMR. Mono-, di-, and trifluoro alpha-methylalanines and their methyl esters have been characterized with respect to their spectra, the pH dependence of their fluorine resonances, and the effects of temperature and cations on the NH2pKa values. These pH indicators can be used singly or in combination to determine intracellular vs. extracellular pH profiles over a wide range (greater than 2 pH units) of extracellular pH.