Determination of mycotoxins produced by Fusarium isolates from banana fruits by capillary gas chromatography and high-performance liquid chromatography
Determination of mycotoxins produced by Fusarium isolates from banana fruits by capillary gas chromatography and high-performance liquid chromatography
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DOI：
10.1016/s0021-9673(97)00328-2
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发表时间：
1997-08-22
影响因子：
4.1
通讯作者：
Mateo, R
中科院分区：
文献类型：
--
作者：
Jimenez, M;Mateo, R
本文介绍了三氯乙烯(雪腐镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、3-和15-乙酰脱氧雪腐镰刀菌烯醇、二乙酰氧基雪腐镰刀菌烯醇、新柳醇、T-2四醇、T-2和HT-2毒素)、玉米赤霉烯酮和玉米赤霉烯醇的分析方法，以及另一种测定伏马菌素B-1的方法，并将其应用于香蕉镰刀菌的培养。这两种方法都适用于这些真菌毒素的提取、净化和测定的不同技术。第一种方法是用甲醇-1%氯化钠水溶液提取，用正己烷和二氯甲烷分配，再用固相反相净化，用高效液相色谱-紫外检测和毛细管气相色谱分析两种洗脱物。伏马菌素B-1的测定方法包括甲醇水提取、浓缩、Amberlite XAD-2柱上水和甲醇净化、形成荧光的4-氟-7-硝基苯并呋喃衍生物、高效液相色谱-荧光检测。这两种方法都提供了良好的检测和回收限度，被认为适合于检测和定量所研究的玉米和水稻镰刀菌培养物中的毒素。从香蕉果实中分离出来。(C)1997年爱思唯尔科学公司。
A method of analysis for trichothecenes (nivalenol, deoxynivalenol, 3- and 15-acetyldeoxynivalenol, diacetoxyscirpenol, neosolaniol, T-2 tetraol, T-2 and HT-2 toxins), zearalenone and zearalenols, and another method for determination of fumonisin B-1 are described and applied to cultures of Fusarium isolated from bananas. Both methods were adapted from different techniques of extraction, clean-up and determination of these mycotoxins. The first method involves extraction with methanol-1% aqueous sodium chloride, clean-up of extracts by partition with hexane and dichloromethane, additional solid reversed-phase clean-up and analysis of two eluates by both high-performance liquid chromatography with ultraviolet detection and capillary gas chromatography. The method for fumonisin B-1 implies extraction with aqueous methanol, concentration, clean-up with water and methanol on Amberlite XAD-2 column, formation of a fluorescent 4-fluoro-7-nitrobenzofurazan derivative and analysis by high-performance liquid chromatography with fluorescence detection. Both procedures give good limits of detection and recoveries, and are considered suitable for the detection and quantification of the studied toxins in corn and rice cultures of Fusarium spp. isolated from banana fruits. (C) 1997 Elsevier Science B.V.