High abundance of JS-1-and Chloroflexi-related Bacteria in deeply buried marine sediments revealed by quantitative, real-time PCR
High abundance of JS-1-and Chloroflexi-related Bacteria in deeply buried marine sediments revealed by quantitative, real-time PCR
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DOI：
10.1111/j.1574-6941.2010.00838.x
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发表时间：
2010-05-01
影响因子：
4.2
通讯作者：
Schippers, Axel
中科院分区：
文献类型：
--
作者：
Blazejak, Anna;Schippers, Axel
在从海洋沉积物中获得的16S rRNA基因克隆文库中，经常可以发现细菌候选组JS-1的成员以及Chloroflei门的AnAerolineae和Caldiineae类成员的序列。使用一种新设计的实时定量聚合酶链式反应分析，这些细菌群在来自秘鲁边缘、黑海和苏门答腊岛附近的弧前盆地的近地表和深埋海洋沉积物的样本中被联合量化。在近表层沉积物中，JS-1以及厌氧菌亚科和甲壳菌亚科相关细菌的16S rRNA基因拷贝数显著低于细菌总数。相比之下，在深埋在约1米以下的沉积物中，发现了这些特定群体和细菌的类似数量的16S rRNA基因拷贝。这一发现表明，JS-1和AnAerolineae和Caldiineae相关细菌可能主导着深埋海洋沉积物中的细菌群落，因此似乎在深层生物圈中发挥着重要的生态作用。
Sequences of members of the bacterial candidate division JS-1 and the classes Anaerolineae and Caldilineae of the phylum Chloroflexi are frequently found in 16S rRNA gene clone libraries obtained from marine sediments. Using a newly designed quantitative, real-time PCR assay, these bacterial groups were jointly quantified in samples from near-surface and deeply buried marine sediments from the Peru margin, the Black Sea, and a forearc basin off the island of Sumatra. In near-surface sediments, sequences of the JS-1 as well as Anaerolineae- and Caldilineae-related Bacteria were quantified with significantly lower 16S rRNA gene copy numbers than the sequences of total Bacteria. In contrast, in deeply buried sediments below approximately 1 m depth, similar quantities of the 16S rRNA gene copies of these specific groups and Bacteria were found. This finding indicates that JS-1 and Anaerolineae- and Caldilineae-related Bacteria might dominate the bacterial community in deeply buried marine sediments and thus seem to play an important ecological role in the deep biosphere.