Mechanical behavior and biochemical composition of canine knee cartilage following periods of joint disuse and disuse with remobilization
Mechanical behavior and biochemical composition of canine knee cartilage following periods of joint disuse and disuse with remobilization
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DOI：
10.1016/s1063-4584(97)80027-1
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发表时间：
1997-01-01
影响因子：
7
通讯作者：
Howell, DS
中科院分区：
文献类型：
--
作者：
Setton, LA;Mow, VC;Howell, DS
在一个关节废用的实验模型中，研究了关节软骨的力学行为和生化成分，在该模型中，犬膝关节以90度屈曲固定在吊带中。在等长拉伸试验中测试股骨表面区域的关节软骨，在压缩压痕试验中测试胫骨平台上的全层软骨。水，蛋白多糖和胶原蛋白的含量进行了测量，在现场匹配的样品。关节废用8周后，以及关节废用4周（远端和近端股骨沟相似，140%）或8周（远端股骨沟相似，140%）后再活动3周后，在软骨中观察到拉伸模量的部位特异性增加（远端股骨沟相似，88%以上的对照值）。与此相反，在压痕试验中测定的软骨的压缩性能与对照值相比没有变化，关节废用或废用后再动员。关节废用8周后，胫骨上某些部位的含水量增加（类似于组织湿重的6%，后部），但股骨表面区域组织中的含水量没有增加。未发现蛋白多糖/胶原和软骨厚度随废用或废用后再动员而变化。在关节废用期后，在股骨的表面区组织（类似于23%，股骨远端沟）和胫骨的全层组织（类似于21%，前部和后部）中观察到蛋白聚糖：水的比值降低。在本研究中，测量的材料性能表明，在停用或停用再动员后，关节面保持完整。这一发现表明了这种关节废用模型与其他实验模型之间的一个重要区别，在其他实验模型中，软骨变化是进行性和退行性的，例如关节炎诱导的关节不稳定。
The mechanical behavior and biochemical composition of articular cartilage were studied in an experimental model of joint disuse, in which the canine knee was immobilized in a sling at 90 degrees of flexion. Articular cartilage from the surface zone of the femur was tested in an isometric tensile test and full-thickness cartilage on the tibial plateau was tested in a compressive indentation test. Water, proteoglycan and collagen contents were measured in site-matched samples. Site-specific increases in the tensile moduli (similar to 88% above control values in distal femoral groove) were observed in cartilage after 8 weeks of joint disuse, and after 3 weeks of remobilization following either 4 (similar to 140%, distal and proximal femoral groove) or 8 weeks (similar to 140%, distal femoral groove) of joint disuse. In contrast, the compressive properties of cartilage deter mined in the indentation test exhibited no change from control values with joint disuse or disuse followed by remobilization. Water contents increased at some sites on the tibia after 8 weeks of joint disuse (similar to 6% of tissue wet weight, posterior site), but not in the surface zone tissue of the femur. Proteoglycan/collagen and cartilage thickness were not found to change with disuse or disuse followed by remobilization. Reduced values for the ratio of proteoglycan:water were observed in the surface zone tissue of the femur (similar to 23%, distal femoral groove) and in the full-thickness tissue of the tibia (similar to 21%, anterior and posterior sites) after periods of joint disuse. In this study, the measured material properties suggest that the articular surface remains intact following periods of disuse or disuse with remobilization. This finding suggests one important difference between this model of joint disuse and other experimental models in which cartilage changes are both progressive and degenerative, such as surgically-induced joint instability.