Laser flow cytometry and cancer chemotherapy: detection of intracellular anthracyclines by flow cytometry.
Laser flow cytometry and cancer chemotherapy: detection of intracellular anthracyclines by flow cytometry.
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激光流式细胞术和癌症化疗：通过流式细胞术检测细胞内蒽环类药物。
DOI：
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发表时间：
1979
影响因子：
3.2
通讯作者：
R. Ganapathi
中科院分区：
文献类型：
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作者：
A. Krishan;R. Ganapathi
属于蒽环类抗生素（例如阿霉素）的重要化疗抗生素的细胞内分布可以通过激光流式细胞术检测。间接方法基于这些化合物干扰碘化丙啶与核 DNA 的结合。在直接方法中，这些抗生素的细胞内荧光在流动系统中被激光束激发和检测。本报告展示了使用这两种方法对许多重要的蒽环类药物进行细胞内检测和定量。
The intracellular distribution of important chemotherapeutic antibiotics belonging to the anthracycline group (e.g. adriamycin) can be detected by laser flow cytometry. The indirect method is based on the interference of these compounds with the binding of propidium iodide to the nuclear DNA. While in the direct method, the intracellular fluorescence of these antibiotics is excited and detected with a laser beam in a flow system. The present report demonstrates the use of these two methods for intracellular detection and quantitation of a number of important anthracyclines.