Evolution of a flipped pathway creates metabolic innovation in tomato trichomes through BAHD enzyme promiscuity.
Evolution of a flipped pathway creates metabolic innovation in tomato trichomes through BAHD enzyme promiscuity.
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DOI：
10.1038/s41467-017-02045-7
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发表时间：
2017-12-12
影响因子：
16.6
通讯作者：
Last RL
中科院分区：
文献类型：
--
作者：
Fan P;Miller AM;Liu X;Jones AD;Last RL
植物通过多步生物合成网络产生数十万种结构多样的专门代谢物，包括具有生态和治疗重要性的化合物。这些途径仅限于特定的植物群，是了解代谢进化的优秀系统。番茄和其他茄科植物在茎和叶的腺毛尖细胞中合成保护性的酰基化糖。我们描述了野生番茄物种的代谢创新，有助于酰基蔗糖结构多样性。两种酰基蔗糖酰基转移酶中少量氨基酸的改变改变了它们对酰基受体的偏好，导致它们的反应顺序颠倒，增加了产物的多样性。该研究证明了多种途径酶中少量氨基酸的变化如何导致植物中特定代谢物的多样化。它还强调了结合遗传学，基因组学和体外生化方法来确定导致代谢新颖性的进化机制的力量。植物通过多步骤途径产生大量结构多样的代谢物，这些途径通常使用相同的前体。在这里，作者利用导致番茄植物中酰基化蔗糖产生的途径来说明代谢物多样性是如何通过生化途径进化产生的。
Plants produce hundreds of thousands of structurally diverse specialized metabolites via multistep biosynthetic networks, including compounds of ecological and therapeutic importance. These pathways are restricted to specific plant groups, and are excellent systems for understanding metabolic evolution. Tomato and other plants in the nightshade family synthesize protective acylated sugars in the tip cells of glandular trichomes on stems and leaves. We describe a metabolic innovation in wild tomato species that contributes to acylsucrose structural diversity. A small number of amino acid changes in two acylsucrose acyltransferases alter their acyl acceptor preferences, resulting in reversal of their order of reaction and increased product diversity. This study demonstrates how small numbers of amino acid changes in multiple pathway enzymes can lead to diversification of specialized metabolites in plants. It also highlights the power of a combined genetic, genomic and in vitro biochemical approach to identify the evolutionary mechanisms leading to metabolic novelty. Plants produce large numbers of structurally diverse metabolites through multistep pathways that often use the same precursors. Here the authors utilize the pathway leading to the production of acylated sucroses in the tomato plant to illustrate how metabolite diversity can arise through biochemical pathway evolution.
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影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
新的先进的番茄番茄种群可以实现高分辨率的叶子QTL映射和基因识别。
DOI：
10.1534/g3.116.030536
发表时间：
2016-10-13
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Fulop D;Ranjan A;Ofner I;Covington MF;Chitwood DH;West D;Ichihashi Y;Headland L;Zamir D;Maloof JN;Sinha NR
通讯作者：
Sinha NR
影响因子：
3.7
作者：
Engler, Carola;Kandzia, Romy;Marillonnet, Sylvestre
通讯作者：
Marillonnet, Sylvestre
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
2.2
作者：
LIEDL, BE;LAWSON, DM;MUTSCHLER, MA
通讯作者：
MUTSCHLER, MA