Peptones and calf serum as a replacement for human serum in the cultivation of Plasmodium falciparum.
Peptones and calf serum as a replacement for human serum in the cultivation of Plasmodium falciparum.
复制标题
在恶性疟原虫培养中，蛋白胨和小牛血清可替代人血清。
DOI：
10.2307/3280810
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
J. Vanderberg
中科院分区：
文献类型：
--
作者：
T. Ifediba;J. Vanderberg
试图找到一种廉价的，容易获得的替代品的人血清的要求，连续培养红细胞期的恶性疟原虫。我们发现，新蛋白胨或蛋白酶-蛋白胨3号与小牛血清一起加入时，寄生虫的生长速度与人血清相当或超过人血清。然而，首先有必要通过逐渐、逐步减少使用的人血清量，同时逐步增加添加的替代品来适应寄生虫。
Attempts were made to find an inexpensive, readily available substitute for human serum requirement for the continuous culture of erythrocytic stages of Plasmodium falciparum. We found that Neopeptone or Proteose-Peptone No. 3 added together with calf serum gave parasite growth rates comparable to, or surpassing, those obtained with human serum. However, first it was necessary to adapt the parasites by a gradual, stepwise reduction in the amount of human serum used, and a concomitant, stepwise increase in the substitutes added.