Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells.
Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells.
复制标题
碱性螺旋-环-螺旋转录因子 DEC2 在紫杉醇诱导的人前列腺癌细胞凋亡过程中充当抗凋亡因子。
DOI：
10.3892/ijmm.2016.2798
复制
发表时间：
2016-12
影响因子：
5.4
通讯作者：
Kijima H
中科院分区：
文献类型：
--
作者：
Liu Q;Wu Y;Yoshizawa T;Yan X;Morohashi S;Seino H;Kato Y;Kijima H
碱性螺旋-环-螺旋（bHLH）转录因子分化的胚胎软骨细胞（DEC）1（BHLHE 40）和2（BHLHE 41）的功能涉及生物节律、免疫应答、细胞增殖、缺氧反应以及恶性肿瘤等多个领域。以前的研究表明，DEC作为各种癌细胞系的凋亡调节剂。然而，关于DEC 1和DEC 2在前列腺癌细胞中的表达知之甚少。本研究旨在研究DEC 1和DEC 2在紫杉醇处理的人前列腺癌DU 145和PC-3细胞中的作用。DEC 1和DEC 2在DU 145细胞中的表达降低，而在PC-3细胞中的表达增加。DU 145细胞对紫杉醇比PC-3细胞更敏感，因为DU 145细胞中裂解的聚（ADP-核糖）聚合酶（PARP）的量在50 μM紫杉醇时达到峰值，而PC-3细胞中在100 μM紫杉醇时达到峰值。此外，在紫杉醇存在下，DEC 1 siRNA降低了切割的PARP的量，而DEC 2 siRNA增加了切割的PARP的量。尽管DEC 2过表达在两种细胞系中轻微抑制了切割的PARP，但DEC 1过表达对细胞凋亡的影响仍有待确定。总之，在紫杉醇诱导的人前列腺癌细胞凋亡中，DEC 1至少部分地发挥了促凋亡作用，而DEC 2发挥了抗凋亡作用。
The functions of basic helix-loop-helix (bHLH) transcription factor-differentiated embryonic chondrocyte (DEC)1 (BHLHE40) and 2 (BHLHE41) are involved in various fields such as circadian rhythms, immune responses, cell proliferation, hypoxia reaction as well as malignant tumors. Previous findings showed that DEC served as apoptosis regulators of various cancer cell lines. However, little is known regarding the expression of DEC1 and DEC2 in prostate cancer cells. The present study aimed to examine the roles of DEC1 and DEC2 in human prostate cancer DU145 and PC-3 cells that were treated with paclitaxel. The expression of DEC1 and DEC2 was decreased in DU145 cells but was increased in PC-3 cells when treated with paclitaxel. DU145 cells were more sensitive to paclitaxel than PC-3 cells since the amount of cleaved poly(ADP-ribose) polymerase (PARP) reached its peak at 50 μM of paclitaxel in DU145 cells but at 100 μM in PC-3 cells. In addition, the amount of cleaved PARP was decreased by DEC1 siRNA, while it was increased by DEC2 siRNA in the presence of paclitaxel. Although DEC2 overexpression slightly inhibited cleaved PARP in the two cell lines, the effects of DEC1 overexpression on apoptosis remain to be determined. In conclusion, DEC1, at least partly, exerted a pro-apoptotic effect, whereas DEC2 exerted an anti-apoptotic effect in paclitaxel-induced apoptosis of human prostate cancer cells.
登录
查看更多内容
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
12.8
作者：
Lim, Soo-Jeong;Choi, Moon Kyung;Kim, Joo Kyoung
通讯作者：
Kim, Joo Kyoung
影响因子：
4.8
作者：
Yamada, K;Kawata, H;Miyamoto, K
通讯作者：
Miyamoto, K
DOI：
10.1073/pnas.070526297
发表时间：
2000-04-11
影响因子：
11.1
作者：
Sun, H;Taneja, R
通讯作者：
Taneja, R
影响因子：
158.5
作者：
Petrylak, DP;Tangen, CM;Crawford, ED
通讯作者：
Crawford, ED