Effects of phenylpropanoid and energetic metabolism inhibition on faba bean resistance mechanisms to rust
Effects of phenylpropanoid and energetic metabolism inhibition on faba bean resistance mechanisms to rust
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DOI：
10.1094/phyto-97-0060
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发表时间：
2007-01-01
期刊：
影响因子：
3.2
通讯作者：
Sillero, Josefina Carmen
中科院分区：
文献类型：
--
作者：
Del Mar Rojas-Molina, Maria;Rubiales, Diego;Sillero, Josefina Carmen
肉桂酸脱氢酶（CAD）自杀抑制剂对透皮吸收和过敏抵抗的影响（[（2-羟基苯基）氨基]亚磺酰基）乙酸，1.1二甲酯，其抑制苯丙素类生物合成，和D-甘露糖，其螯合磷酸盐并减少宿主细胞中可用的能量，研究了蚕豆（Vicia faba）对蚕豆锈病（Uromyces viciae-fabae）具有不同抗性机制的基因型。CAD的抑制降低了品系2N-34、2N-52、V-1271和V-1272的穿透抗性，揭示了苯丙烷类生物合成在这些品系的抗性中的重要作用。CAD的抑制也抑制了这些细胞系中的过敏性细胞死亡。D-甘露糖对抗性的影响很小或没有影响。相比之下，CAD抑制不影响品系BPL-261的穿透阻力，其具有与过敏性细胞死亡无关的高度穿透阻力。在BPL-261中，D-甘露糖抑制渗透阻力。相似的抑制剂处理后，蚕豆的基因型反应锈病观察到这里和大麦基因型的反应与不同的抗性机制白粉病之间的平行性进行了分析和讨论。
Effects on penetration and hypersensitive resistance of the cinnamyl acid dehydrogenase (CAD) suicide inhibitor ([(2-hydroxyphenyl) amino] sulphinyl) acetic acid, 1.1 dimethyl ester, which suppresses phenylpropanoid biosynthesis, and of D-mannose, which sequesters phosphate and reduces energy available in host cells, were studied in faba bean (Vicia faba) genotypes with differing resistance mechanisms to faba bean rust (Uromyces viciae-fabae). Inhibition of CAD reduced penetration resistance in lines 2N-34, 2N-52, V-1271, and V-1272, revealing an important role for phenylpropanoid biosynthesis in the resistance of these lines. Inhibition of CAD also inhibited hypersensitive cell death in these lines. D-mannose had little or no effect on resistance. By contrast, CAD inhibition did not affect penetration resistance of line BPL-261, which has a high degree of penetration resistance not associated with hypersensitive cell death. In BPL-261, D-mannose inhibited penetration resistance. The parallelism between the faba bean genotype responses to rust observed here and the response of barley genotypes with differing resistance mechanisms to powdery mildew after similar inhibitor treatments is analyzed and discussed.