Characterization of a Female-Produced Courtship Pheromone in the Parasitoid Nasonia vitripennis
Characterization of a Female-Produced Courtship Pheromone in the Parasitoid Nasonia vitripennis
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DOI：
10.1007/s10886-006-9102-3
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发表时间：
2006-08
影响因子：
2.3
通讯作者：
S. Steiner;Nadin Hermann;J. Ruther
中科院分区：
文献类型：
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作者：
S. Steiner;Nadin Hermann;J. Ruther
丽翅蜂Nasonia vitripennis（步行者）（膜翅目：金小蜂科）的雄蜂具有典型的求偶行为。我们证明，男性的吸引力和求爱序列的关键行为元素介导的女性衍生的接触性信息素。男性被逮捕的纸盘与女性提取物处理，但不与男性提取物处理。男性的反应性受到女性提取物应用的表面的影响。提取物适用于提取甲虫elytron逮捕男性更强烈地比那些适用于滤纸的大小相当。然而，更复杂的行为元素，如点头和交配的尝试，只显示提取物被应用到提取的男性尸体，这表明触觉或视觉线索协同男性的反应。所涉及的化学品是稳定的、低挥发性的和非极性的。死亡雌虫能使雄虫产生至少8d的求偶行为。男性没有表现出任何迹象的吸引力活女性在3厘米的距离在嗅觉仪。雌性提取物的分馏表明，活性仅位于非极性馏分中。通过气相色谱-质谱分析的活性馏分显示，存在具有25和37个碳单元之间的链长的表皮烃。对男性和女性的碳氢化合物特征进行比较，发现在质量和数量上存在差异。这些结果表明，性特异性表皮碳氢化合物是介导雄性求偶行为的关键信号。丽翅凤蝶
Males of the parasitoidNasonia vitripennis(Walker) (Hymenoptera: Pteromalidae) show a characteristic courtship behavior. We demonstrate that male arrestment and key behavioral elements of the courtship sequence are mediated by a female-derived contact sex pheromone. Males were arrested on paper disks treated with female extracts but not on those treated with male extracts. Male responsiveness was influenced by the surface to which female extracts were applied. Extracts applied to an extracted beetle elytron arrested males more strongly than those applied to filter paper of comparable size. However, more complex behavioral elements, such as head nodding and copulation attempts, were shown only when extracts were applied to extracted male cadavers, suggesting that tactile or visual cues synergize the male response. The chemicals involved are stable, of low volatility, and nonpolar. Dead females arrested males and elicited courtship behavior for at least 8 d. Males showed no sign of attraction to live females at a distance of 3 cm in an olfactometer. Fractionation of female extracts demonstrated that the activity was exclusively located in the nonpolar fraction. Analysis of the active fraction by gas chromatography–mass spectrometry revealed that cuticular hydrocarbons with chain lengths between 25 and 37 carbon units were present. Comparison of hydrocarbon profiles from males and females showed qualitative and quantitative differences. These results suggest that sex-specific cuticular hydrocarbons are the key signals mediating the male courtship behavior inN. vitripennis.