The Distribution and Cellular Lineages of XX and XY Cells in Gonads Associated with Ovotesticular Disorder of Sexual Development.
The Distribution and Cellular Lineages of XX and XY Cells in Gonads Associated with Ovotesticular Disorder of Sexual Development.
复制标题
与卵睾性发育障碍相关的性腺中 XX 和 XY 细胞的分布和细胞谱系。
作者：
Nishina-Uchida N;Fukuzawa R;Ishii T;Anaka MR;Hasegawa T;Hasegawa Y.
具有46、XX/46、XY核型的个体被归类为卵睾丸性发育障碍（ODSD），其性腺要么一侧为卵巢，另一侧为睾丸，要么为混合卵睾丸。为检测3例ODSD患者生殖腺46、XX和46、XY细胞的分布，采用FISH检测X、Y染色体，免疫组化检测SOX9和FOXL2。FISH分析显示，在精小管的Sertoli细胞中存在XX信号，而在卵泡上皮细胞中发现含有Y信号的细胞。无论是否存在反向性染色体，在精管和卵泡中，SOX9和FOXL2的免疫标记是相互排斥的。因此，我们认为个体性腺上皮细胞的命运不仅由性染色体决定，而且由局部环境因素决定。
Individuals with a 46, XX/46, XY karyotype are categorized as ovotesticular disorder of sexual development (ODSD) and have gonads with either an ovary on one side and a testis on the other side or a mixed ovotestis. To examine the distribution of 46, XX and 46, XY cells in gonads of 3 patients with ODSD, FISH for X and Y chromosomes and immunohistochemistry for SOX9 and FOXL2 were carried out. FISH analysis showed that XX signals were present in Sertoli cells in the seminiferous tubules, while cells containing Y signals were seen in epithelia of ovarian follicles. The immunolabeling of SOX9 and FOXL2 in the seminiferous tubules and ovarian follicles was mutually exclusive, irrespective of the presence of reversed sex chromosomes. We therefore suggest that the fate of individual gonadal epithelial cells is determined not only by the sex chromosomes but also by local environmental factors.