A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
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一种简单制备高比活32P标记三磷酸腺苷的方法
DOI：
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发表时间：
1964
期刊：
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通讯作者：
J. Chappell
中科院分区：
文献类型：
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作者：
I. Glynn;J. Chappell
描述了通过交换反应制备高比活性的/sup 32/P-标记的ATP的方法。该方法简单，仅需要市售的底物和酶。用重粒肌球蛋白水解标记的 ATP 表明 98% 至 99% 的 /sup 32/P 位于 1-磷酸基团中。 （授权）
A method is described for preparing /sup 32/P-labeled ATP of high specific activity by an excbange reaction. The method is simple and requires only substrates and enzymes that are available commercially. Hydrolysis of the labeled ATP with heavy meromyosin indicates that 98 to 99% of the /sup 32/P is in the 1 -phosphate group. (auth)