MINOCYCLINE PREVENTS THE IMPAIRMENT OF HIPPOCAMPAL LONG-TERM POTENTIATION IN THE SEPTIC MOUSE
MINOCYCLINE PREVENTS THE IMPAIRMENT OF HIPPOCAMPAL LONG-TERM POTENTIATION IN THE SEPTIC MOUSE
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DOI：
10.1097/shk.0000000000000847
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发表时间：
2017-08-01
期刊：
影响因子：
3.1
通讯作者：
Morimoto, Yuji
中科院分区：
文献类型：
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作者：
Hoshino, Koji;Hayakawa, Mineji;Morimoto, Yuji
脓毒症相关性脑病是脓毒症的主要并发症，有效的治疗方法仍然未知。虽然米诺环素（MINO）具有神经保护作用，是治疗脓毒症相关脑病的有吸引力的候选药物，但MINO对脓毒症期间突触可塑性的影响仍不清楚。在本研究中，我们研究了MINO对盲肠结扎穿孔（CLP）小鼠模型海马长时程增强（LTP）的影响。我们将小鼠分为四组：假手术+溶媒、假手术+ MINO（60 mg/kg，在切片制备前连续3天腹腔注射）、CLP+溶媒和CLP+MINO。我们使用术后24小时的切片测试了海马CA 1区的LTP。由于MINO也具有抗炎作用，因此在IL-1受体拮抗剂（IL-1 ra）灌注30分钟后分析LTP。CLP术后24 h，无论是否给予MINO，血液中的内毒素水平均升高，CLP +溶媒组小鼠的LTP严重受损（P
Sepsis-associated encephalopathy is a major complication during sepsis, and an effective treatment remains unknown. Although minocycline (MINO) has neuroprotective effects and is an attractive candidate for treating sepsis-associated encephalopathy, the effect of MINO on synaptic plasticity during sepsis is still unclear. In the present study, we investigated the effects of MINO on long-term potentiation (LTP) in the hippocampus in a cecal ligation and puncture (CLP) mouse model. We divided mice into four groups; sham + vehicle, sham + MINO (60 mg/kg, i.p. for 3 consecutive days before slice preparation), CLP+vehicle, and CLP+MINO. We tested LTP in the CA1 region of the hippocampus, using slices taken 24 h after surgery. Because MINO is also anti-inflammatory, LTP was analyzed following 30 min of IL-1 receptor antagonist (IL-1ra) perfusion. The endotoxin level in the blood was increased at 24 h after CLP operations regardless of MINO administrations, and LTP in the CLP + vehicle group mice was severely impaired (P