Identification, cloning and sequencing of Escherichia coli strain KH1378 (O78:K80) iss gene isolated from poultry colibacillosis in Iran
Identification, cloning and sequencing of Escherichia coli strain KH1378 (O78:K80) iss gene isolated from poultry colibacillosis in Iran
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DOI：
10.1111/j.1472-765x.2009.02681.x
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发表时间：
2009-09-01
影响因子：
2.4
通讯作者：
Karimi, V.
中科院分区：
文献类型：
--
作者：
Derakhshandeh, A.;Salehi, T. Zahraei;Karimi, V.
目的：从大肠杆菌中鉴定、克隆并测序血清存活率增高基因（iss）.方法：从伊朗分离的大肠杆菌KH 1378菌株中克隆iss基因，并对其蛋白产物进行预测。coli KH 1378中扩增出目的基因，克隆到pTZ 57 R/T载体上，并进行序列测定。从DNA序列中，使用生物信息学评估Iss预测蛋白。KH 1378菌株的Iss与其它E.大肠埃希菌血清型和不同来源的分离株的同源性也高达98%。coliO 157：H7 Iss蛋白。系统发育分析表明，E.结论：预测的Iss蛋白在不同的大肠杆菌菌株间存在较强的相关性。表明该抗原可作为禽源致病性大肠杆菌的控制和检测抗原。研究的意义和影响：由于确切的发病机制和毒力因子的作用尚不清楚，Iss蛋白可作为未来疫苗接种的靶点，但需要进一步研究。
Aims:To identify, clone and sequence the iss (increased serum survival) gene from E. coli strain KH1378 isolated from Iranian poultry and to predict its protein product, Iss.Methods and Results:The iss gene from E. coli strain KH1378 was amplified and cloned into the pTZ57R/T vector and sequenced. From the DNA sequence, the Iss predictive protein was evaluated using bioinformatics. Iss from strain KH1378 had 100% identity with other E. coli serotypes and isolates from different origins and also 98% identity with E. coli O157:H7 Iss protein. Phylogenetic analysis showed no significant different phylogenic groups among E. coli strains.Conclusions:The strong association of predicted Iss protein among different E. coli strains suggests that it could be a good antigen to control and detect avian pathogenic E. coli (APEC).Significance and Impact of the study:Because the exact pathogenesis and the role of virulence factors are unknown, the Iss protein could be used as a target for vaccination in the future, but further research is required.