Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column
Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column
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DOI：
10.1152/ajpregu.00254.2006
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发表时间：
2007-02-01
影响因子：
2.8
通讯作者：
Itoh, Hiroshi
中科院分区：
文献类型：
--
作者：
Iigaya, Kamon;Kumagai, Hiroo;Itoh, Hiroshi
从延髓腹外侧尾端到中间外侧细胞柱的新轴突投射。Am J Physiol Regul Integr Comp Physiol 292：R927-R936，2007年。首次发表于2006年11月2日; doi：10.1152/ajpregu.00254.2006。我们使用的光学成像技术，以调查是否神经元的轴突在尾端的腹外侧延髓（CeVLM），以及轴突的神经元在头端腹外侧延髓（RVLM），项目的神经元在中间外侧细胞柱（IML）的脊髓。用电压敏感染料对来自新生血压正常的Wistar-Kyoto和自发性高血压大鼠的脑干-脊髓制备物进行染色，并且用光学成像装置（MiCAM-01）检测对Th-2水平的IML的电刺激的响应，作为荧光强度的变化。结果如下：（1）在低钙高镁灌流时，在延髓腹侧面的CeVLM水平和RVLM水平均检测到对IML刺激的去极化反应;（2）在横截面的CeVLM水平也检测到去极化反应，其潜伏期为24.0 ± 5.5（SD）ms，3）在CeVLM神经元中检测到对IML刺激的逆向动作电位，其中检测到光学图像。4）向CeVLM施加谷氨酸增加兴奋性突触后电位（EPSP）的频率并诱导IML神经元的去极化。光学成像结果表明，一种新的轴突和功能的投射从CeVLM的IML的神经元。对谷氨酸作用的反应表明，CeVLM参与了交感神经活动和血压的调节，并可能与尾加压区相对应。
Novel axonal projection from the caudal end of the ventrolateral medulla to the intermediolateral cell column. Am J Physiol Regul Integr Comp Physiol 292: R927-R936, 2007. First published November 2, 2006; doi: 10.1152/ajpregu.00254.2006.-We used an optical imaging technique to investigate whether axons of neurons in the caudal end of the ventrolateral medulla (CeVLM), as well as axons of neurons in the rostral ventrolateral medulla (RVLM), project to neurons in the intermediolateral cell column (IML) of the spinal cord. Brain stem-spinal cord preparations from neonatal normotensive Wistar-Kyoto and spontaneously hypertensive rats were stained with a voltage- sensitive dye, and responses to electrical stimulation of the IML at the Th-2 level were detected as changes in fluorescence intensity with an optical imaging apparatus (MiCAM-01). The results were as follows: 1) depolarizing responses to IML stimulation during low-Ca high-Mg superfusion were detected on the ventral surface of the medulla at the level of the CeVLM, as well as at the level of the RVLM, 2) depolarizing responses were also detected on cross sections at the level of the CeVLM, and they had a latency of 24.0 +/- 5.5 (SD) ms, 3) antidromic action potentials in response to IML stimulation were demonstrated in the CeVLM neurons where optical images were detected, and 4) glutamate application to the CeVLM increased the frequency of excitatory postsynaptic potentials (EPSPs) and induced depolarization of the IML neurons. The optical imaging findings suggested a novel axonal and functional projection from neurons in the CeVLM to the IML. The increase in EPSPs of the IML neurons in response to glutamate application suggests that the CeVLM participates in the regulation of sympathetic nerve activity and blood pressure and may correspond to the caudal pressor area.