Practical aspects of NMR signal assignment in larger and challenging proteins.
Practical aspects of NMR signal assignment in larger and challenging proteins.
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DOI：
10.1016/j.pnmrs.2013.12.001
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发表时间：
2014-04
影响因子：
6.1
通讯作者：
Frueh, Dominique P.
中科院分区：
文献类型：
--
作者：
Frueh, Dominique P.
关键词：
核磁共振技术已经发展成为一种在接近生理条件下研究蛋白质的常规技术。然而，分配NMR信号所需的各种相关图的复杂性阻碍了对较大蛋白质的应用。本文回顾了传统上用于共振分配的数据分析技术，并描述了克服大蛋白质谱挑战所需的替代方案。特别地，多个光谱的同时分析可以帮助克服模糊性或者可以以间接方式揭示相关性。类似地，多维光谱中的正交平面的可视化可以提供替代的分配过程。我们描述了这样的策略分配的骨干，甲基和nOe共振的例子。我们描述了相关实验的数据采集实验方面，并提供初步研究的指导方针。重点放在大折叠单体蛋白质和37，48，53和81 kDa的蛋白质提供的例子。
NMR has matured into a technique routinely employed for studying proteins in near physiological conditions. However, applications to larger proteins are impeded by the complexity of the various correlation maps necessary to assign NMR signals. This article reviews the data analysis techniques traditionally employed for resonance assignment and describes alternative protocols necessary for overcoming challenges in large protein spectra. In particular, simultaneous analysis of multiple spectra may help overcome ambiguities or may reveal correlations in an indirect manner. Similarly, visualization of orthogonal planes in a multidimensional spectrum can provide alternative assignment procedures. We describe examples of such strategies for assignment of backbone, methyl, and nOe resonances. We describe experimental aspects of data acquisition for the related experiments and provide guidelines for preliminary studies. Focus is placed on large folded monomeric proteins and examples are provided for 37, 48, 53, and 81 kDa proteins.
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影响因子：
2.7
作者：
Chou, JJ;Baber, JL;Bax, A
通讯作者：
Bax, A
DOI：
10.1073/pnas.0610313104
发表时间：
2007-06-05
影响因子：
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影响因子：
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作者：
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