A two-dimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining
A two-dimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining
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DOI：
10.1002/elps.200406010
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发表时间：
2004-08-01
期刊：
影响因子：
2.9
通讯作者：
Lee, KH
中科院分区：
文献类型：
--
作者：
Hayduk, EJ;Choe, LH;Lee, KH
我们报告了基于荧光染色和串联飞行时间 (TOF/TOF) 质谱法的中国仓鼠卵巢 (CHO) 细胞蛋白详细二维电泳图谱的开发。即使 CHO 基因组尚未测序，我们仍观察到蛋白质鉴定的成功率为 71%。该图谱由 274 个二维凝胶电泳 (2-DE) 凝胶点中存在的 224 个蛋白质组成。我们还使用市售的磷蛋白特异性荧光染色剂启动了一项磷蛋白组研究。使用这种染色剂，我们观察到 672 个磷酸化蛋白质，包括许多已知被磷酸化的蛋白质，占我们用总蛋白质染色剂可视化的蛋白质的 36%，与预期一致。
We report on the development of a detailed two-dimensional electrophoresis map of Chinese hamster ovary (CHO) cell proteins based on fluorescence staining and tandem time-of-flight (TOF/TOF)-mass spectrometry. We observed a 71% success rate in the identification of proteins even though the CHO genome is not sequenced. The map consists of 224 protein identifications present in 274 two-dimensional gel electrophoresis (2-DE) gel spots. We have also initiated a study of the phosphoproteome using a commercially available phosphoprotein-specific fluorescent stain. Using this stain, we observe 672 phosphorylated proteins, including many proteins known to be phosphorylated, which is 36% of the proteins we visualized with a total protein stain and consistent with expectations.