Molecular characterization of XVSAP1, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker
Molecular characterization of XVSAP1, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker
复制标题
DOI：
10.1093/jxb/54.381.191
复制
发表时间：
2003-01-01
影响因子：
6.9
通讯作者：
Mundree, SG
中科院分区：
文献类型：
--
作者：
Garwe, D;Thomson, JA;Mundree, SG
采用“功能互补”的策略，从脱水粘旱生植物叶片cDNA文库中分离到一个cDNA，命名为XVSAP 1。cDNA序列分析表明，具有六个跨膜区域的高度疏水性蛋白。Southern杂交分析表明，XVSAP 1在X.粘的。推导的氨基酸序列与小麦低温调节蛋白WCOR 413的同源性为49%。该蛋白还显示出与来自拟南芥的WCOR 413样蛋白的25%至56%的同一性。XVSAP 1在大肠杆菌（srl：：Tn 10）中的表达赋予细胞在1 M山梨醇中生长时的渗透胁迫耐受性。利用半定量RT-PCR分析基因表达表明，XVSAP 1是由脱水，盐胁迫（100 mM），低温（4 ℃）和高温（42 ℃）和强光处理（1500 mumol m（-2）s（-1））诱导的。这些结果表明XVSAP 1可能在X. viscosa对非生物胁迫的反应
The strategy of 'complementation by functional sufficiency' was used to isolate a cDNA designated XVSAP1 from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low-temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. Expression of XVSAP1 in Escherichia coli (srl::Tn10) conferred osmotic stress tolerance when the cells were grown in 1 M sorbitol. Analysis of gene expression using semi-quantitative RT-PCR indicated that XVSAP1 is induced by dehydration, salt stress (100 mM), both low (4 degreesC) and high temperature (42 degreesC) and high light treatment (1500 mumol m(-2) s(-1)). These results suggest that XVSAP1 may have a significant role to play in the response of X. viscosa to abiotic stresses.