Ionic matrices pre-spotted matrix-assisted laser desorption/ionization plates for patient maker following in course of treatment, drug titration, and MALDI mass spectrometry imaging
Ionic matrices pre-spotted matrix-assisted laser desorption/ionization plates for patient maker following in course of treatment, drug titration, and MALDI mass spectrometry imaging
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DOI：
10.1016/j.ab.2012.10.035
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发表时间：
2013-03-01
影响因子：
2.9
通讯作者：
Fournier, Isabelle
中科院分区：
文献类型：
--
作者：
Bonnel, David;Franck, Julien;Fournier, Isabelle
在目前的研究中，我们比较了塑料基质辅助激光解吸/电离（MALDI）板预点不同的固体离子基质。数据表明，储存3个月后，标准品在α-氰基-4-羟基肉桂酸（HCCA）中氧化，无论是否在HCCA/3-乙酰基吡啶（3APY）和HCCA/苯胺中，使用HCCA基质未检测到某些肽（如泛素），而在预点样离子基质中检测到。这些MALDI基质预点样板（储存3个月后）与卵巢囊肿液在肽组学中的应用显示，与固体离子基质相比，HCCA的信号强度较低。我们表明，这些预先点样的离子基质板可用于相对药物定量，高质量蛋白质检测和MALDI质谱成像。(c)2012 Elsevier Inc. All rights reserved.
In the current study, we compared plastic matrix-assisted laser desorption/ionization (MALDI) plates pre-spotted with different solid ionic matrices. Data reflect that after 3 months of storage, the standards were oxidized in alpha-cyano-4-hydroxycinnamic acid (HCCA) whether or not in HCCA/3-acetylpyridine (3APY) and HCCA/aniline, and certain peptides, such as ubiquitin, were not detected using the HCCA matrix, whereas they were detected in pre-spotted ionic matrices. Application in peptidomics of these MALDI matrices pre-spotted plates (after 3 months of storage) with ovarian cyst fluid showed less intense signals with HCCA than with solid ionic matrices. We show that these pre-spotted ionic matrices plates can be used for relative drug quantification, high mass protein detection, and MALDI mass spectrometry imaging. (c) 2012 Elsevier Inc. All rights reserved.