"Parahomologous" gene targeting in Drosophila cells: an efficient, homology-dependent pathway of illegitimate recombination near a target site.
"Parahomologous" gene targeting in Drosophila cells: an efficient, homology-dependent pathway of illegitimate recombination near a target site.
复制标题
果蝇细胞中的“准同源”基因靶向：靶位点附近非法重组的有效、同源依赖性途径。
DOI：
10.1093/genetics/145.2.349
复制
发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Cherbas,P
中科院分区：
文献类型：
--
作者：
Cherbas,L;Cherbas,P
Drosophila cells in culture can be transformed by introducing exogenous DNA carrying a selectable marker. Here we report on the fate of plasmids that contain an extended fragment of Drosophila DNA in addition to the selectable marker. A small minority of the resulting transformants appear to arise from homologous recombination at the chromosomal target. However, the majority of the insertions are the products of illegitimate events in the vicinity of the target DNA, and they often cause mutations in the targeted region. The efficiency of this process, its homology dependence, and the clustering of the products define a novel transformation pathway that we call “parahomologous targeting.”
