Salinity-induced changes in gene expression from anterior and posterior gills of Callinectes sapidus (Crustacea: Portunidae) with implications for crustacean ecological genomics.
Salinity-induced changes in gene expression from anterior and posterior gills of Callinectes sapidus (Crustacea: Portunidae) with implications for crustacean ecological genomics.
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DOI：
10.1016/j.cbd.2016.06.002
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发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Santos SR
中科院分区：
文献类型：
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作者：
Havird JC;Mitchell RT;Henry RP;Santos SR
十足类动物是甲壳类动物中最具生态多样性的分类群之一，使它们成为研究诸如呼吸调节等生理过程的理想动物。然而，以前的研究没有考虑到整个转录组的反应鳃-主要器官负责离子运输-改变盐度。此外，分子遗传差异之间的非调节性和调节性鳃类型，以及激素的基础上调节性鳃，仍然未充分探索。在这里，我们通过RNA-Seq确定和表征了在蓝蟹Callinectes sapidus（一种研究良好的甲壳类动物盐度调节模型）中从高到低的盐度转移过程中前（非盐度调节）和后（盐度调节）鳃中的差异表达基因（DEG）。总体而言，我们证实了以前的表达模式的个别离子转运基因，并确定了新的盐度介导的表达。值得注意的是，新的DEG之间的盐度和鳃类型的C。sapidus包括肌动蛋白和角质层蛋白等结构基因表达较高的前鳃，而后鳃则表现出离子转运和能量相关基因的表达升高，后者可能与离子转运有关。回收DEG之间的激素调节盐度和鳃型离子运输的潜在目标包括神经肽Y和KCTD 16样蛋白。使用可获得的序列数据，成分的“核心”鳃十足类动物之间的转录组，包括参与离子运输和能量转换的基因，并与盐度转移实验。最后，稀疏分析使我们推荐适度数量的序列读段（~10-15 M），但具有增加的生物复制，用于甲壳类动物的未来DEG分析。
Decapods represent one of the most ecologically diverse taxonomic groups within crustaceans, making them ideal to study physiological processes like osmoregulation. However, prior studies have failed to consider the entire transcriptomic response of the gill – the primary organ responsible for ion transport – to changing salinity. Moreover, the molecular genetic differences between non-osmoregulatory and osmoregulatory gill types, as well as the hormonal basis of osmoregulation, remain underexplored. Here, we identified and characterized differentially expressed genes (DEGs) via RNA-Seq in anterior (non-osmoregulatory) and posterior (osmoregulatory) gills during high to low salinity transfer in the blue crab Callinectes sapidus, a well-studied model for crustacean osmoregulation. Overall, we confirmed previous expression patterns for individual ion transport genes and identified novel ones with salinity-mediated expression. Notable, novel DEGs among salinities and gill types for C. sapidus included anterior gills having higher expression of structural genes such as actin and cuticle proteins while posterior gills exhibit elevated expression of ion transport and energy-related genes, with the latter likely linked to ion transport. Potential targets among recovered DEGs for hormonal regulation of ion transport between salinities and gill types included neuropeptide Y and a KCTD16-like protein. Using publically available sequence data, constituents for a “core” gill transcriptome among decapods are presented, comprising genes involved in ion transport and energy conversion and consistent with salinity transfer experiments. Lastly, rarefication analyses lead us to recommend a modest number of sequence reads (~10–15 M), but with increased biological replication, be utilized in future DEG analyses of crustaceans.