Camelid nanobodies: killing two birds with one stone
Camelid nanobodies: killing two birds with one stone
复制标题
DOI：
10.1016/j.sbi.2015.01.001
复制
发表时间：
2015-06-01
影响因子：
6.8
通讯作者：
Cambillau, Christian
中科院分区：
文献类型：
--
作者：
Desmyter, Aline;Spinelli, Silvia;Cambillau, Christian
近年来，单结构域骆驼免疫球蛋白，称为vHH或纳米体，在生物技术、药物应用和结构/功能研究中的应用日益增长。纳米小体在结构生物学中的有用性现在已经得到证实，因为它们提供了在凹面和铰链区域获得新表位的途径，并使它们稳定下来。这些位点通常与酶抑制或受体中和有关，同时也为晶体堆积提供了有利的表面。通过使用具有柔性多结构域蛋白的纳米体、大的复合体以及最后但并非最不重要的膜蛋白，已经取得了显著的结果。虽然生成纳米体仍然是一个相当漫长和昂贵的过程，但朴素的库的出现可能会促进整个过程。
In recent years, the use of single-domain camelid imnnunoglobulins, termed vHHs or nanobodies, has seen increasing growth in biotechnology, pharmaceutical applications and structure/function research. The usefulness of nanobodies in structural biology is now firmly established, as they provide access to new epitopes in concave and hinge regions and stabilize them. These sites are often associated with enzyme inhibition or receptor neutralization, and, at the same time, provide favorable surfaces for crystal packing. Remarkable results have been achieved by using nanobodies with flexible multi-domain proteins, large complexes and, last but not least, membrane proteins. While generating nanobodies is still a rather long and expensive procedure, the advent of naive libraries might be expected to facilitate the whole process.