Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger
Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger
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DOI：
10.1139/cjm-47-1-9
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发表时间：
2001-01-01
影响因子：
2.8
通讯作者：
Dargent, R
中科院分区：
文献类型：
--
作者：
de Billerbeck, VG;Roques, CG;Dargent, R
结果表明，香茅（Cymbopogon nardus（L.）W.沃森变种在琼脂培养基上测定了罗勒精油对尼日尔（货车Tieghem）菌丝的抑制作用。800 mg/L时菌丝生长完全被抑制。发现该浓度在试验条件下是致命的。400 mg/L的精油在培养4天后引起80%的生长抑制，并且与对照相比，分生孢子延迟4天。通过显微镜观察，确定了A.黑尼日尔菌丝经C. Nardus精油透射电镜观察到的主要变化是菌丝直径和菌丝壁明显变薄。这些细胞学结构的改变可能是由于精油干扰了负责细胞壁合成的酶，从而干扰了正常的生长。此外，精油引起质膜破坏和线粒体结构紊乱。因此，研究结果表明，开发香茅精油作为生物降解和存储污染真菌的有效抑制剂的可能性。
The growth inhibitory effect of Cymbopogon nardus (L.) W. Watson var. nardus essential oil on Aspergillus niger (Van Tieghem) mycelium was determined on agar medium. The mycelium growth was completely inhibited at 800 mg/L. This concentration was found to be lethal under the test conditions. Essential oil at 400 mg/L caused growth inhibition of 80% after 4 days of incubation, and a delay in conidiation of 4 days compared with the control. Microscopic observations were carried out to determine the ultrastructural modifications of A. niger hyphae after treatment with C. nardus essential oil. The main change observed by transmission electron microscopy concerned the hyphal diameter and the hyphal wall, which appeared markedly thinner. These modifications in cytological structure might be caused by the interference of the essential oil with the enzymes responsible for wall synthesis which disturb normal growth. Moreover, the essential oil caused plasma membrane disruption and mitochondrial structure disorganization. The findings thus indicate the possibility of exploiting Cymbopogon nardus essential oil as an effective inhibitor of biodegrading and storage-contaminating fungi.