Activation of endoplasmic reticulum stress response following trauma-hemorrhage.
Activation of endoplasmic reticulum stress response following trauma-hemorrhage.
复制标题
DOI：
10.1016/j.bbadis.2008.08.007
复制
发表时间：
2008-11
影响因子：
6.2
通讯作者：
Raju, Raghavan
中科院分区：
文献类型：
--
作者：
Jian, Bixi;Hsieh, Chi-Hsun;Chen, Jianguo;Choudhry, Mashkoor;Bland, Kirby;Chaudry, Irshad;Raju, Raghavan
出血性创伤导致器官功能障碍、败血症和死亡。库普弗细胞异常产生促炎细胞因子、组织缺氧和创伤出血后的肝损伤。创伤出血引起的生理条件与启动内质网（ER）应激和未折叠蛋白反应所需的因素一致。然而，内质网应激对创伤出血后细胞凋亡和肝损伤的影响尚不清楚。在本研究中，我们对经历创伤出血或假手术的小鼠进行了内质网应激的研究。与对照组相比，创伤出血后肝脏中内质网应激蛋白 Bip、ATF6、PERK、IRE1α 和 PDI 的表达显着升高。创伤出血组内质网应激相关的促凋亡转录因子CHOP蛋白表达也显着升高。与此一致的是，在创伤出血后的肝脏中观察到 DNA 断裂增强，证实了细胞凋亡。这些结果证明了内质网应激的启动及其在出血性创伤后细胞凋亡和肝损伤中的作用。
Hemorrhagic trauma leads to organ dysfunction, sepsis and death. There is abnormal production of proinflammatory cytokines by Kupffer cells, tissue hypoxia and liver injury following trauma-hemorrhage. The physiological conditions consequent to trauma-hemorrhage are consistent with factors necessary to initiate endoplasmic reticulum (ER) stress and unfolded protein response. However, the contribution of ER stress to apoptosis and liver injury after trauma-hemorrhage is not known. In the present study ER stress was investigated in mice that underwent trauma-hemorrhage or sham operation. Expression of endoplasmic reticulum stress proteins Bip, ATF6, PERK, IRE1α, and PDI were significantly elevated in the liver after trauma-hemorrhage compared to the controls. The ER stress associated proapoptotic transcription factor CHOP protein expression was also significantly elevated in trauma-hemorrhage group. Consistent with this, enhanced DNA fragmentation was observed, confirming apoptosis, in the liver following trauma-hemorrhage. These results demonstrate the initiation of ER stress and its role in apoptosis and liver injury, subsequent to hemorrhagic trauma.
登录
查看更多内容
DOI：
10.1152/ajpgi.00217.2002
发表时间：
2003-01-01
影响因子：
4.5
作者：
Ma, YC;Wang, P;Messina, JL
通讯作者：
Messina, JL
影响因子：
2.2
作者：
Higuchi, Shinya;Wu, Rongqian;Wang, Ping
通讯作者：
Wang, Ping
影响因子：
3.8
作者：
AYALA, A;PERRIN, MM;CHAUDRY, IH
通讯作者：
CHAUDRY, IH
影响因子：
5
作者：
Hsieh, Ya-Ching;Yu, Huang-Ping;Chaudry, Irshad H.
通讯作者：
Chaudry, Irshad H.
影响因子：
3.1
作者：
Frink, Michael;Hsieh, Ya-Ching;Chaudry, Irshad H.
通讯作者：
Chaudry, Irshad H.