MicroRNA528, a hub regulator modulating ROS homeostasis via targeting of a diverse set of genes encoding copper-containing proteins in monocots
MicroRNA528, a hub regulator modulating ROS homeostasis via targeting of a diverse set of genes encoding copper-containing proteins in monocots
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MicroRNA528，一种中枢调节剂，通过靶向单子叶植物中编码含铜蛋白的多种基因来调节 ROS 稳态
DOI：
10.1111/nph.16130
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发表时间：
2020
期刊：
影响因子：
9.4
通讯作者：
Xia Rui
中科院分区：
文献类型：
--
作者：
Zhu Hong;Chen Chengjie;Zeng Jun;Yun Ze;Liu Yuanlong;Qu Hongxia;Jiang Yueming;Duan Xuewu;Xia Rui
植物microRNA（miRNAs）调节重要的细胞过程，包括对极端温度的反应，而极端温度通常与活性氧（ROS）密切相关。在本研究中，发现异常温度引起香蕉果实中大量miRNAs的丰度发生广泛变化，特别是铜（Cu）相关miRNAs。其中，miR 528在冷胁迫下显著下调，并且发现其靶向编码多酚氧化酶（PPO）的基因，与在水稻和玉米中鉴定的那些不同。PPO基因的表达在低温条件下上调超过100倍，导致ROS激增和随后的香蕉果实果皮布朗宁。广泛的比较基因组分析表明，单子叶植物特异性miR 528可以潜在地靶向编码含铜蛋白的大量基因。miR 528不仅是ROS产生酶，而且也是ROS清除酶，参与细胞内ROS的代谢; miR 528在不同单子叶植物中具有明显的靶基因偏好性，其靶位点在不同基因家族中的位置不同，这表明miR 528是一个高度动态的靶基因多样化过程。其靶向编码含铜蛋白的基因的广泛能力暗示miR 528是调节单子叶植物细胞ROS稳态的关键调节因子。
Plant microRNAs (miRNAs) regulate vital cellular processes, including responses to extreme temperatures with which reactive oxygen species (ROS) are often closely associated.In the present study, it was found that aberrant temperatures caused extensive changes in abundance to numerous miRNAs in banana fruit, especially the copper (Cu)‐associated miRNAs. Among them, miR528 was significantly downregulated under cold stress and it was found to target genes encoding polyphenol oxidase (PPO), different from those identified in rice and maize. Expression ofPPOgenes was upregulated by > 100‐fold in cold conditions, leading to ROS surge and subsequent peel browning of banana fruit.Extensive comparative genomic analyses revealed that the monocot‐specific miR528 can potentially target a large collection of genes encoding Cu‐containing proteins. Most of them are actively involved in cellular ROS metabolism, including not only ROS generating oxidases, but also ROS scavenging enzymes.It also was demonstrated that miR528 has evolved a distinct preference of target genes in different monocots, with its target site varying in position among/within gene families, implying a highly dynamic process of target gene diversification. Its broad capacity to target genes encoding Cu‐containing protein implicates miR528 as a key regulator for modulating the cellular ROS homeostasis in monocots.