Potential therapeutic applications of decoy oligonucleotides.
Potential therapeutic applications of decoy oligonucleotides.
复制标题
诱饵寡核苷酸的潜在治疗应用。
DOI：
--
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
T. Ogihara
中科院分区：
文献类型：
--
作者：
N. Tomita;R. Morishita;T. Tomita;T. Ogihara
分子生物学的最新进展提供了抑制靶基因表达的新技术。特别是，应用DNA技术，如反义寡核苷酸（ODN）策略来调节体内疾病相关基因的转录，具有显着的治疗潜力。近年来，转染双链寡核苷酸（诱饵寡核苷酸）作为一种新的强有力的工具，在一类新的抗基因策略的基因治疗。对应于顺式序列的双链ODN的转染将导致真实的顺式-反式相互作用的减弱，导致从内源性顺式元件中去除反式因子，随后调节基因表达。
Recent progress in molecular biology has provided new techniques with which to inhibit target gene expression. In particular, the application of DNA technologies, such as antisense oligonucleotide (ODN) strategies to regulate the transcription of disease-related genes in vivo, have significant therapeutic potential. Recently, transfection of cis-element double-stranded oligonucleotides (decoy ODNs) has been reported as a new powerful tool in a new class of anti-gene strategies for gene therapy. Transfection of double-stranded ODN corresponding to the cis sequence will result in attenuation of the authentic cis-trans interaction, leading to removal of trans-factors from the endogenous cis-elements with subsequent modulation of gene expression.