Chemical concentration measurement in blood serum and urine samples using liquid-core optical fiber Raman spectroscopy
Chemical concentration measurement in blood serum and urine samples using liquid-core optical fiber Raman spectroscopy
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DOI：
10.1364/ao.46.001726
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发表时间：
2007-04-01
期刊：
影响因子：
1.9
通讯作者：
Berger, Andrew J.
中科院分区：
文献类型：
--
作者：
Qi, Dahu;Berger, Andrew J.
我们报道了使用液芯光纤(LCOF)拉曼光谱测量临床血清和尿样中的化学浓度，以增加收集的信号强度。利用LCOF几何结构获得了近红外区的拉曼光谱和吸收光谱。通过偏最小二乘法对71份血清和61份尿样的光谱与参考值进行了回归。13种化学物质的预测浓度和参考浓度之间存在显著的相关性。使用吸收数据对LCOF增强进行归一化处理，使结果更加准确。该实验几何结构非常适合对透明的生物流体进行大容量和自动化的化学分析。(C)2007年美国光学学会。
We report measurements of chemical concentrations in clinical blood serum and urine samples using liquid-core optical fiber (LCOF) Raman spectroscopy to increase the collected signal strength. Both Raman and absorption spectra were acquired in the near-infrared region using the LCOF geometry. Spectra of 71 blood serum and 61 urine samples were regressed via partial least squares against reference analyzer values. Significant correlation was found between predicted and reference concentrations for 13 chemicals. Using absorption data to normalize the LCOF enhancement made the results more accurate. The experimental geometry is well suited for high-volume and automated chemical analysis of clear biofluids. (c) 2007 Optical Society of America.