In vitro antioxidant activities of three selected brown seaweeds of India
In vitro antioxidant activities of three selected brown seaweeds of India
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DOI：
10.1016/j.foodchem.2007.08.081
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发表时间：
2008-03-15
期刊：
影响因子：
8.8
通讯作者：
Bhaskar, N.
中科院分区：
文献类型：
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作者：
Chandini, S. Kumar;Ganesan, P.;Bhaskar, N.
三种选定的印度褐海藻的体外抗氧化活性-即，调查了缘边马尾藻、四口莲座蕨和锥状喇叭藻。对粗甲醇提取物的总酚含量和还原力进行了研究。总甲醇提取物和五个不同的级分（即，石油醚（PE）、乙酸乙酯（EA）、二氯甲烷（DCM）、丁醇（BuOH）和水溶液）的总抗氧化活性、DPPH自由基清除和脱氧核糖测定。S.在所有级分中，边藻表现出较高的总抗氧化活性，为39.62 mg抗坏血酸当量/g提取物（或0.31 mg抗坏血酸当量/g海藻，以干重计）。不同海藻提取物中，S.边叶木的PE组分清除DPPH自由基的活性最高，为23.16%;芋螺的脱氧核糖酶活力较低，为47.81%。结果表明，海藻水提物中酚类物质含量较高（49.16mg没食子酸当量/g提取物或0.86mg GAE/g海藻干重）;圆锥形。甲醇粗提物的还原力随浓度的增加而增加。还原力T.与标准抗氧化剂（α-生育酚）相比，芋螺和四口扁螺的抗氧化性更高。在海藻中，T.芋螺酚含量显著高于其它两种（P < 0.05）。在体外抗氧化活性的甲醇提取物从所有三种海藻表现出增加的浓度，表明这些属性的剂量依赖性。(C)2007爱思唯尔有限公司保留所有权利。
In vitro antioxidant activities of three selected Indian brown seaweeds - viz., Sargassum marginatum, Padina tetrastomatica and Turbinaria conoides - were investigated. Total phenolic content and reducing power of crude methanolic extract were also investigated. The activity of total methanolic extract and five different fractions (viz., petroleum ether (PE), ethyl acetate (EA), dichloromethane (DCM), butanol (BuOH) and aqueous) were studied using total antioxidant activity, DPPH radical scavenging and deoxyribose assays. EA fraction of S. marginatum exhibited higher total antioxidant activity of 39.62 mg ascorbic acid equivalent/g extract (or 0.31 mg ascorbic acid equivalent/g seaweed on dry weight basis) among the all the fractions. Among the fractions obtained from different seaweeds, EA fraction of S. marginatum showed higher DPPH scavenging activity of 23.16%; while PE fraction of T. conoides exhibited lower deoxyribose activity of 47.81%. Higher phenolic content (49.16 mg gallic acid equivalent/g extract or 0.86 mg GAE/g of seaweed on dry weight basis) was noticed in aqueous fraction of T. conoides. Reducing power of crude methanolic extract increased with increasing concentration. Reducing power of T. conoides and P. tetrastomatica were higher compared to standard antioxidant (alpha-tocopherol). Among the seaweeds, total methanolic extract of T. conoides had significantly higher phenol content (P < 0.05) compared to the other two species. In vitro antioxidant activity of methanolic extracts from all the three seaweeds showed an increase with increasing concentration indicating the dose dependency of these properties. (C) 2007 Elsevier Ltd. All rights reserved.