Expression of vascular endothelial growth factor and hypoxia-inducible factor in the airway of asthmatic patients
Expression of vascular endothelial growth factor and hypoxia-inducible factor in the airway of asthmatic patients
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DOI：
10.1016/s1081-1206(10)60971-4
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发表时间：
2006-12-01
影响因子：
5.9
通讯作者：
Kim, Young Kyoon
中科院分区：
文献类型：
--
作者：
Lee, Sook Young;Kwon, Soon;Kim, Young Kyoon
背景：血管内皮生长因子（VEGF）是一种强有力的促血管生成细胞因子，它也增加血管通透性。众所周知，哮喘患者气道中VEGF水平升高。缺氧诱导因子（HIF）诱导VEGF表达迅速而强烈的增加。目的：探讨哮喘患者VEGF表达水平与临床特征的关系，并确定VEGF表达与HIF表达是否相关。采用酶联免疫吸附法检测支气管肺泡灌洗液（BALF）中VEGF的含量。我们测量VEGF，HIF-1 α，HIF-2 α的表达活检标本通过免疫反应性。结果：VEGF水平在支气管哮喘患者的BALF中显着高于对照组。哮喘患者BALF中VEGF水平与嗜酸性粒细胞计数呈正相关（r = 0.501; P <0.01）。VEGF水平与1秒用力呼气量百分比和气道高反应性无关。与对照组相比，哮喘患者的粘膜下层VEGF、HIF-1 α和HIF-2 α的免疫反应性更高。此外，VEGF表达与HIF-1 α（r = 0.614; P = 0.02）和HIF-2 α（r = 0.881; P = 0.001）表达显著相关。结论：这些发现表明VEGF可能在炎症中发挥重要作用，并且支气管哮喘中VEGF水平与HIF有关。
Background: Vascular endothelial growth factor (VEGF) is a potent proangiogenic cytokine, and it also increases vascular permeability. It is well known that VEGF levels are increased in the airway of asthmatic patients. Hypoxia-inducible factor (HIF) induces a rapid and strong increase in VEGF expression.Objectives: To evaluate the relationship between VEGF level and clinical characteristics and to determine whether VEGF expression is associated with HIF expression in asthmatic patients.Methods: Bronchoscopy was performed on 30 asthmatic patients and 14 control subjects. The concentration of VEGF in the bronchoalveolar lavage fluid (BALF) was examined using enzyme-linked immuncisorbent assay. We measured VEGF, HIF-1 alpha, and HIF-2 alpha expression on biopsy specimens by means of immunoreactivity.Results: The VEGF level in the BALF was significantly higher in asthmatic patients than in controls. The VEGF level correlated with eosinophil counts in the BALF in asthmatic subjects (r = 0.501; P < .01). However, the VEGF level did not correlate with percentage of forced expiratory volume in 1 second and airway hyperresponsiveness. Asthmatic patients exhibited higher VEGF, HIF-1 alpha, and HIF-2 alpha immunoreactivity in the submucosa than did controls. Furthermore, VEGF expression correlated significantly with HIF-1 alpha (r = 0.614; P = .02) and HIF-2 alpha (r = 0.881; P = .001) expression.Conclusion: These findings suggest that VEGF may play an important role in inflammation and that VEGF level is related to HIF in bronchial asthma.