Folding Analytical Devices for Electrochemical ELISA in Hydrophobic RH Paper
Folding Analytical Devices for Electrochemical ELISA in Hydrophobic RH Paper
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DOI：
10.1021/ac5020782
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发表时间：
2014-12-16
影响因子：
7.4
通讯作者：
Whitesides, George M.
中科院分区：
文献类型：
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作者：
Glavan, Ana C.;Christodouleas, Dionysios C.;Whitesides, George M.
这项工作描述了一种用于电化学酶联免疫吸附测定（ELISA）的设备，该设备设计用于低资源环境和诊断。该装置完全在疏水纸中制造，通过用癸基三氯硅烷化纸来生产，并且包括由中心折痕分开的两个区：压花微孔，在其表面上发生抗原或抗体固定和识别事件，以及印刷电极的检测区。通过沿该中心折痕沿着折叠装置使两个区域接触;从折叠构型记录分析信号。两个概念验证的应用程序，电化学直接ELISA检测兔IgG作为模型抗原在缓冲液中和电化学夹心ELISA检测疟疾富含组氨酸的蛋白质从恶性疟原虫（Pf HRP 2）在加标的人血清中，显示该设备的多功能性。定量加标人血清中Pf HRP 2的电化学夹心ELISA检测限为4 ng mL（-1）（10（2）pmol L-1），该值在临床相关浓度范围内。
This work describes a device for electrochemical enzyme-linked immunosorbent assay (ELISA) designed for low-resource settings and diagnostics at the point of care. The device is fabricated entirely in hydrophobic paper, produced by silanization of paper with decyl trichlorosilane, and comprises two zones separated by a central crease: an embossed microwell, on the surface of which the antigen or antibody immobilization and recognition events occur, and a detection zone where the electrodes are printed. The two zones are brought in contact by folding the device along this central crease; the analytical signal is recorded from the folded configuration. Two proof-of-concept applications, an electrochemical direct ELISA for the detection of rabbit IgG as a model antigen in buffer and an electrochemical sandwich ELISA for the detection of malarial histidine-rich protein from Plasmodium falciparum (Pf HRP2) in spiked human serum, show the versatility of this device. The limit of detection of the electrochemical sandwich ELISA for the quantification of Pf HRP2 in spiked human serum was 4 ng mL(-1) (10(2) pmol L-1), a value within the range of clinically relevant concentrations.