Impact of metal exposure on environmentally isolated Serratia marcescens' growth, oxidative-stress resistance, biofilm formation, and proliferation in eukaryotic co-culture models
Impact of metal exposure on environmentally isolated Serratia marcescens' growth, oxidative-stress resistance, biofilm formation, and proliferation in eukaryotic co-culture models
复制标题
DOI：
10.1016/j.ecoenv.2023.114677
复制
发表时间：
2023-02-24
影响因子：
6.8
通讯作者：
Rosenzweig，Jason A.
中科院分区：
文献类型：
--
作者：
Adedoyin，Folasade T.;Sridhar，Balaji Bhaskar Maruthi;Rosenzweig，Jason A.
环境金属可能对周围的生物群有害，间接影响淡水栖息地，也影响微生物群落。在这项研究中，锌（Zn）（55.5 mg/kg），锰（Mn）（863.4 mg/kg）和铅（Pb）（17.5 mg/kg）在休斯顿流域洪泛平原土壤样品中测得的水平高于环境机构的阈值。为了探讨金属暴露的影响，环境isolatedSerratia marcescens（SME），心内膜炎和呼吸道感染的病原体，其参考菌株（SMR）暴露于铅，锌，锰，随后的氧化应激反应和生物膜的生产进行了测量。毫不奇怪，SME对所有3种金属暴露的敏感性低于SMR。有趣的是，SME产生增加的生物膜，更耐氧化应激的存在下，锌和铅比SMR。在使用BAES-2B细胞的6小时肺部感染模型中，SME在所有金属挑战中表现出比SMR更大的增殖。类似地，在我们的HT 29肠道感染模型中，当在6小时感染后用Pb和Mn挑战时，SME增殖超过SMR。总之，SME比SMR更能承受环境压力，这表明这种机会性人类病原体的毒力潜力增加。
Environmental metals can be noxious to the surrounding biota, indirectly impact freshwater habitats, and also impact microbiological communities. In this study, zinc (Zn) (55.5 mg/kg), manganese (Mn) (863.4 mg/kg) and lead (Pb) (17.5 mg/kg) levels measured in Houston watershed flood plain soil samples were higher than environmental agencies’ thresholds. To investigate the effects of metal exposures, an environmentally isolatedSerratia marcescens(SME), etiological agent of endocarditis and respiratory infections, and its reference strain (SMR) were exposed to Pb, Zn, and Mn, and subsequent oxidative stress responses and biofilm production were measured. Not surprisingly, SME was less sensitive to all 3 metal exposures than was SMR. Interestingly, SME produced increased biofilm and was more resistant to oxidative stress in the presence of Zn and Pb than SMR. In a 6 h lung infection model using BAES-2B cells, SME exhibited greater proliferation than SMR in all metal challenges. Similarly, in our HT29 gut infection model, SME out-proliferated SMR when challenged with Pb and Mn following the 6 h infection. Taken together, SME was better able to withstand environmental stressors than SMR, suggesting increased virulence potential of this opportunistic human pathogen.