Molecular evolution analysis and geographic investigation of severe acute respiratory syndrome coronavirus-like virus in palm civets at an animal market and on farms
Molecular evolution analysis and geographic investigation of severe acute respiratory syndrome coronavirus-like virus in palm civets at an animal market and on farms
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DOI：
10.1128/jvi.79.18.11892-11900.2005
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发表时间：
2005-09-01
影响因子：
5.4
通讯作者：
Xu, JG
中科院分区：
文献类型：
--
作者：
Kan, B;Wang, M;Xu, JG
2004年1月，在中国广东省市售动物中发现SARS冠状病毒后，为消除严重急性呼吸系统综合症（SARS）再次出现的来源，扑杀了大量棕榈果子狸。在扑杀前取样的所有91只来自动物市场的棕榈果子狸和15只浣熊中发现了该病毒，但后来在分布在12个省的25个农场取样的1107只棕榈果子狸中未发现该病毒，这些农场被声称是交易动物的来源。基于本研究和其他已发表的研究中获得的17个动物序列，在刺突基因上鉴定出27个新的特征变异残基（snv），并分析了它们的系统发育关系。分析表明，活禽市场棕榈果子狸中的病毒已经进化到感染人类。进化的起点是一个由三个动物起源的病毒序列组成的原型群。最初，7个SNV位点在刺突蛋白的147、228、240、479、821和1080位点引起6个氨基酸变化，以产生低致病性病毒。其中之一与2003-2004年期间的第一位SARS患者有关。另外14个snv引起了11个氨基酸残基的变化，分别位于360、462、472、480、487、609、613、665、743、765和1163位。由此产生的高致病性群体导致了2003年所谓的早期流行病期间的感染。最后，剩下的6个snv引起了227、244、344和778位4个氨基酸的变化，从而形成了导致全球流行的病毒群。
Massive numbers of palm civets were culled to remove sources for the reemergence of severe acute respiratory syndrome (SARS) in Guangdong Province, China, in January 2004, following SARS coronavirus detection in market animals. The virus was identified in all 91 palm civets and 15 raccoon dogs of animal market origin sampled prior to culling, but not in 1,107 palm civets later sampled at 25 farms, spread over 12 provinces, which were claimed to be the source of traded animals. Twenty-seven novel signature variation residues (SNVs) were identified on the spike gene and were analyzed for their phylogenetic relationships, based on 17 sequences obtained from animals in our study and from other published studies. Analysis indicated that the virus in palm civets at the live-animal market had evolved to infect humans. The evolutionary starting point was a prototype group consisting of three viral sequences of animal origin. Initially, seven SNV sites caused six amino acid changes, at positions 147, 228, 240, 479, 821, and 1080 of the spike protein, to generate low-pathogenicity viruses. One of these was linked to the first SARS patient in the 2003-2004 period. A further 14 SNVs caused 11 amino acid residue changes, at positions 360, 462, 472, 480, 487, 609, 613, 665, 743, 765, and 1163. The resulting high-pathogenicity groups were responsible for infections during the so-called early-phase epidemic of 2003. Finally, the remaining six SNVs caused four amino acid changes, at positions 227, 244, 344, and 778, which resulted in the group of viruses responsible for the global epidemic.