GLYCOSYLINOSITOL PHOSPHOLIPID ANCHORS OF THE SCRAPIE AND CELLULAR PRION PROTEINS CONTAIN SIALIC-ACID
GLYCOSYLINOSITOL PHOSPHOLIPID ANCHORS OF THE SCRAPIE AND CELLULAR PRION PROTEINS CONTAIN SIALIC-ACID
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DOI：
10.1021/bi00136a600
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发表时间：
1992-06-02
期刊：
影响因子：
2.9
通讯作者：
PRUSINER, SB
中科院分区：
文献类型：
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作者：
STAHL, N;BALDWIN, MA;PRUSINER, SB
瘙痒病朊病毒中唯一被识别的成分是 PrP(Sc)，这是一种糖基肌醇磷脂 (GPI) 连接蛋白，通过迄今未知的翻译后事件衍生自细胞亚型 (PrP(C))。对 PrP(Sc) GPI 的分析揭示了六种不同的糖型，其中三种是前所未有的。其中两种糖型含有 N-乙酰神经氨酸，此前从未报道过它是任何 GPI 的组成部分。 GPI 的最大形式被认为具有由 Man-α-Man-α-Man-(NeuAc-Gal-GalNAc-)Man-GlcN-Ino 组成的聚糖核心。在两种不同的朊病毒分离株或“菌株”中发现了相同的 PrP(Sc) GPI 结构，它们指定了叙利亚仓鼠大脑中不同的孵化时间、神经病理学和 PrP(Sc) 分布。对 PrP(C) GPI 的有限分析表明，它也具有唾液酸化糖型，认为这种单糖的存在并不能区分 PrP(C) 和 PrP(Sc)。
The only identified component of the scrapie prion is PrP(Sc), a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrP(C)) by an as yet unknown posttranslational event. Analysis of the PrP(Sc) GPI has revealed six different glycoforms, three of which are unprecedented. Two of the glycoforms contain N-acetylneuraminic acid, which has not been previously reported as a component of any GPI. The largest form of the GPI is proposed to have a glycan core consisting of Man-alpha-Man-alpha-Man-(NeuAc-Gal-GalNAc-)Man-GlcN-Ino. Identical PrP(Sc) GPI structures were found for two distinct isolates or "strains" of prions which specify different incubation times, neuropathology, and PrP(Sc) distribution in brains of Syrian hamsters. Limited analysis of the PrP(C) GPI reveals that it also has sialylated glycoforms, arguing that the presence of this monosaccharide does not distinguish PrP(C) from PrP(Sc).