A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha
A Method for Evaluating Three-Dimensional Morphological Features: A Case Study Using Marchantia polymorpha
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DOI：
10.3389/fpls.2019.01214
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发表时间：
2019-10-02
影响因子：
5.6
通讯作者：
Tsukaya, Hirokazu
中科院分区：
文献类型：
--
作者：
Furuya, Tomoyuki;Kimori, Yoshitaka;Tsukaya, Hirokazu
形态学特征的描述和评价对许多生物学研究至关重要。生物成像和定量方法已经发展到分析植物的形态特征。然而，由于具有相同基因型个体的复杂器官结构具有很大的灵活性，有效的三维成像及其定量研究仍处于发展阶段，特别是在植物形态学研究中。在这项研究中，我们提出了一种新的方法，该方法结合了使用微计算机断层扫描的3D成像技术和数学图像处理方法来描述3D形态特征。以陆地植物进化研究的新模式植物多形地豆（Marchantia polymorpha）为例，对其三维形态异常的突变个体进行了评价。利用这一新方法，定量描述了多态稻的菌体形态，并区分了具有不同形态特征的野生型和突变型。我们新建立的方法可以应用于具有不规则三维形态的各种组织或身体。
The description and evaluation of morphological features are essential to many biological studies. Bioimaging and quantification methods have been developed to analyze the morphological features of plants. However, efficient three-dimensional (3D) imaging and its quantification are still under development, particularly for studies of plant morphology, due to complex organ structure with great flexibility among individuals with the same genotype. In this study, we propose a new approach that combines a 3D imaging technique using micro-computed tomography and a mathematical image-processing method to describe 3D morphological features. As an example, we applied this method to Marchantia polymorpha, a new model plant used for the evolutional study of land plants, and we evaluated a mutant individual with an abnormal 3D shape. Using this new method, we quantitatively described the thallus morphology of M. polymorpha and distinguished the wild type from a mutant with different morphological features. Our newly established method can be applied to various tissues or bodies with irregular 3D morphology.